Articles
ANTIOXIDANT AND ANTIRADICAL ACTIVITY OF LEAVES OF POLYCARPAEA CORYMBOSA IN VITRO
Article number
841_74
Pages
523 – 528
Language
English
Abstract
Reactive oxygen species (ROS) are continuously produced during normal physiologic events, but are eliminated through antioxidant defense mechanisms.
Under pathological conditions, ROS are over-produced and results in oxidative stress.
Antioxidants have been reported to prevent this oxidative stress and thereby prevent the occurrence of diseases like cancer and aging.
Flavonoids and phenolic compounds widely distributed in plants have been reported to exert multiple biological effects, including antioxidant, anti-inflammatory, anticarcinogenic, etc.
The leaves of the plant Polycorpaea corymbosa (family Caryophyllaceae) are used in Indian traditional medicinal system in inflammatory swellings and in treatment of jaundice (Chopra et al., 1956). When plant extracts were subjected to general phytochemical screening, they have shown good amount of phenolics.
Since the plant is used in the treatment of jaundice and inflammation in the traditional system of medicine, the presence of phenolics in the plant inspired us to study its antioxidant properties.
The antioxidant potency of hydro-alcoholic extract of Polycarpaea corymbosa (PC) was investigated employing various established in vitro systems.
IC50 values were determined in each experiment.
Further, total antioxidant capacity and total phenolic content of PC was determined.
IC50 values of PC were found to be 15.10±0.10, 207.96±32.77, 134.47±21.39, 18.03±3.22, 46.73±3.39 µg/ml in DPPH radical scavenging, NO scavenging, B-CLAMS, .OH radical scavenging and anti-lipid peroxidation activity, respectively.
The phytochemical investigation revealed the presence of flavonoids.
Acute toxicity study was also carried out to know whether it has toxicity.
PC was found to be safe at a maximum dose of 5000 mg/kg body wt.
The results indicate that PC possesses potent antioxidant activity, and the presences of flavonoids are found to be responsible for this activity.
Under pathological conditions, ROS are over-produced and results in oxidative stress.
Antioxidants have been reported to prevent this oxidative stress and thereby prevent the occurrence of diseases like cancer and aging.
Flavonoids and phenolic compounds widely distributed in plants have been reported to exert multiple biological effects, including antioxidant, anti-inflammatory, anticarcinogenic, etc.
The leaves of the plant Polycorpaea corymbosa (family Caryophyllaceae) are used in Indian traditional medicinal system in inflammatory swellings and in treatment of jaundice (Chopra et al., 1956). When plant extracts were subjected to general phytochemical screening, they have shown good amount of phenolics.
Since the plant is used in the treatment of jaundice and inflammation in the traditional system of medicine, the presence of phenolics in the plant inspired us to study its antioxidant properties.
The antioxidant potency of hydro-alcoholic extract of Polycarpaea corymbosa (PC) was investigated employing various established in vitro systems.
IC50 values were determined in each experiment.
Further, total antioxidant capacity and total phenolic content of PC was determined.
IC50 values of PC were found to be 15.10±0.10, 207.96±32.77, 134.47±21.39, 18.03±3.22, 46.73±3.39 µg/ml in DPPH radical scavenging, NO scavenging, B-CLAMS, .OH radical scavenging and anti-lipid peroxidation activity, respectively.
The phytochemical investigation revealed the presence of flavonoids.
Acute toxicity study was also carried out to know whether it has toxicity.
PC was found to be safe at a maximum dose of 5000 mg/kg body wt.
The results indicate that PC possesses potent antioxidant activity, and the presences of flavonoids are found to be responsible for this activity.
Publication
Authors
V.I. Hukkeri, M. Kenganora
Keywords
reactive oxygen species, ROS, DPPH, B-CLAMS, flavonoids, phenolics, jaundice
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