Articles
DIVERSITY OF VEGETATIVE COMPATIBILITY TYPES, MATING TYPES AND OCCURRENCE OF HYPOVIRULENCE IN CRYPHONECTRIA PARASITICA POPULATIONS IN NW SPAIN
Article number
844_53
Pages
381 – 386
Language
English
Abstract
A survey was carried out to study the population biology of Cryphonectria parasitica in chestnut forests of Lugo and Ourense provinces (NW Spain), estimating the vegetative compatibility (vc) type and mating type diversity, and the occurrence of hypovirulent strains.
A total of 610 isolates of C. parasitica were obtained, most showing a virulent phenotype.
Only four isolates, from Lugo province, showed the presence of dsRNA, which was indicative of hypovirulence.
Hypovirus molecular characterization of these isolates showed that all belonged to subtype E of Cryphonectria hypovirus 1 (CHV1-E). The diversity of vc types was very low.
Only 9 vc types were found, being five of them present in both provinces.
Four vc types were compatible with the European testers EU1, EU9, EU66 and EU72, and the other five corresponded to new undescribed types, named E1, E2, E5, E6 and E7. The dominant vc types in both populations were E1, E2 and E5. Two hypovirulent strains were compatible with vc type E1, one with vc type E2 and one with vc type E5. The study of mating types showed that the idiomorph MAT-1 was predominant in both populations.
A total of 610 isolates of C. parasitica were obtained, most showing a virulent phenotype.
Only four isolates, from Lugo province, showed the presence of dsRNA, which was indicative of hypovirulence.
Hypovirus molecular characterization of these isolates showed that all belonged to subtype E of Cryphonectria hypovirus 1 (CHV1-E). The diversity of vc types was very low.
Only 9 vc types were found, being five of them present in both provinces.
Four vc types were compatible with the European testers EU1, EU9, EU66 and EU72, and the other five corresponded to new undescribed types, named E1, E2, E5, E6 and E7. The dominant vc types in both populations were E1, E2 and E5. Two hypovirulent strains were compatible with vc type E1, one with vc type E2 and one with vc type E5. The study of mating types showed that the idiomorph MAT-1 was predominant in both populations.
Publication
Authors
O. Aguín, D. Montenegro, M. Hermida, J.P. Mansilla, M.J. Sainz
Keywords
biological control, Castanea sativa, chestnut blight, CHV1 subtype, dsRNA virus
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