Articles
EFFECTS OF NITROGEN AND SULFUR APPLICATION ON ANTIOXIDANT SUBSTANCES IN LEAF MUSTARD
Article number
856_10
Pages
83 – 90
Language
English
Abstract
Leaf mustard (Brassica juncea Coss) has been widely used freshly and processed in southern China and contains natural antioxidants, which are closely associated with human health.
A field experiment was established to investigate the effects of nitrogen and sulfur fertilization on antioxidant substances in two cultivars of leaf mustard (Xuelihong and Baobaoqingcai), including four levels of nitrogen (10.3, 100, 200 and 300 kg/ha) and two levels of sulfur (0 and 50 kg/ha). The results showed that increasing nitrogen application increased the content of
-carotene, but when the level of nitrogen was higher than 200 kg/ha, their contents remained nearly constant.
Sulfur treatments had no significant effects on-carotene.
Ascorbic acid (AsA) content in leaf mustard firstly increased, and then decreased with increasing nitrogen supply, however, the glutathione (GSH) content increased persistently.
Sulfur application increased the AsA and GSH content in the two cultivars of leaf mustard.
Total phenolics decreased with increasing nitrogen application, the highest content was observed when the level of nitrogen was 100 kg/ha, at the same time total phenolics increased by increasing sulfur supply.
Quercetin and kaempferol were identified in leaf mustard, and kaempferol content was 2.46 times higher than quercetin.
The quercetin in Xuelihong increased with increasing nitrogen supply, however, the quercetin content in Baobaoqingcai decreased gradually.
The kaempferol content was highest when the level of nitrogen was 100 kg/ha.
Sulfur application increased the quercetin content in both cultivars, but increased the kaempferol content in Xuelihong only.
There was significant interaction between nitrogen and sulfur on AsA and GSH, but not on β-carotene and phenolic substances.
These results suggested the antioxidant substances in leaf mustard were strongly affected by fertilizer regimes especially nitrogen and sulfur application.
A field experiment was established to investigate the effects of nitrogen and sulfur fertilization on antioxidant substances in two cultivars of leaf mustard (Xuelihong and Baobaoqingcai), including four levels of nitrogen (10.3, 100, 200 and 300 kg/ha) and two levels of sulfur (0 and 50 kg/ha). The results showed that increasing nitrogen application increased the content of
-carotene, but when the level of nitrogen was higher than 200 kg/ha, their contents remained nearly constant.Sulfur treatments had no significant effects on
-carotene.Ascorbic acid (AsA) content in leaf mustard firstly increased, and then decreased with increasing nitrogen supply, however, the glutathione (GSH) content increased persistently.
Sulfur application increased the AsA and GSH content in the two cultivars of leaf mustard.
Total phenolics decreased with increasing nitrogen application, the highest content was observed when the level of nitrogen was 100 kg/ha, at the same time total phenolics increased by increasing sulfur supply.
Quercetin and kaempferol were identified in leaf mustard, and kaempferol content was 2.46 times higher than quercetin.
The quercetin in Xuelihong increased with increasing nitrogen supply, however, the quercetin content in Baobaoqingcai decreased gradually.
The kaempferol content was highest when the level of nitrogen was 100 kg/ha.
Sulfur application increased the quercetin content in both cultivars, but increased the kaempferol content in Xuelihong only.
There was significant interaction between nitrogen and sulfur on AsA and GSH, but not on β-carotene and phenolic substances.
These results suggested the antioxidant substances in leaf mustard were strongly affected by fertilizer regimes especially nitrogen and sulfur application.
Authors
J. Li, Z.J. Zhu, S.R. Guo
Keywords
Brassica juncea Coss, β-carotene, ascorbic acid, glutathione, phenolic, nitrogen, sulfur
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