Articles
HPLC IDENTIFICATION OF GLUCOSINOLATES IN CHINESE KALE
Article number
856_24
Pages
181 – 186
Language
English
Abstract
Glucosinolates (GSs) are sulfur-containing compounds that upon hydrolysis by myrosinase produce several products.
Some compounds have the function of cancer-prevention.
In this paper, glucosinolate composition and contents in 28 Chinese kale cultivars, a traditional Chinese Brassica vegetable, were studied.
Glucosinolates can be separated successfully in HPLC system with gradient eluents.
Eight kinds of glucosinolates were detected in Chinese kale.
Among the 28 Chinese kale cultivars, 04BF-1229-1 had the highest total glucosinolate content, which was 319.46 μmol100 g-1 FW, followed by Zizhi (285.46 µmol100 g-1 FW) and Dongfangzhengda No.1 (274.34 μmol100 g-1 FW). The lowest total GSs content was 32.48 μmol100 g-1 FW with cultivar No.66. Glucosinolate predominant composition and content varied among different Chinese kale cultivars.
Gluconapin was the highest content GS in most of the cultivars.
Glucoraphanin, the direct precursor of sulforaphane, had the highest content in cultivar DE, which was 49.59 μmol 100 g-1 FW, while cultivar Sijiyouqing had the highest percentage of Glucoraphanin to total GSs content which was 36.5%.
Some compounds have the function of cancer-prevention.
In this paper, glucosinolate composition and contents in 28 Chinese kale cultivars, a traditional Chinese Brassica vegetable, were studied.
Glucosinolates can be separated successfully in HPLC system with gradient eluents.
Eight kinds of glucosinolates were detected in Chinese kale.
Among the 28 Chinese kale cultivars, 04BF-1229-1 had the highest total glucosinolate content, which was 319.46 μmol100 g-1 FW, followed by Zizhi (285.46 µmol100 g-1 FW) and Dongfangzhengda No.1 (274.34 μmol100 g-1 FW). The lowest total GSs content was 32.48 μmol100 g-1 FW with cultivar No.66. Glucosinolate predominant composition and content varied among different Chinese kale cultivars.
Gluconapin was the highest content GS in most of the cultivars.
Glucoraphanin, the direct precursor of sulforaphane, had the highest content in cultivar DE, which was 49.59 μmol 100 g-1 FW, while cultivar Sijiyouqing had the highest percentage of Glucoraphanin to total GSs content which was 36.5%.
Authors
M. Song, S.R. Guo, H.J. He, W. Liu
Keywords
Brassica vegetables, secondary metabolite, bioactive compounds, sulforaphane, genotype
Online Articles (37)