Articles
SIMULTANEOUS ISOLATION AND IDENTIFICATION OF POLYMORPHIC SIMPLE SEQUENCE REPEATS
Article number
859_1
Pages
23 – 29
Language
English
Abstract
DNA-based markers have revolutionized the whole process of generating genetic maps, as well as parentage analysis, association mapping, marker assisted selection and the genetic fingerprinting of cultivars and associated population analysis.
Modern molecular techniques have been developed to meet the demands of the agriculture and horticulture industries.
A major bottleneck in the application of molecular techniques to the identification and improvement of plants is the development of the appropriate molecular markers for every species.
The most popular source of markers is the simple sequence repeats (SSRs) found in all eukaryotic genomes.
However, since the primers to characterize the polymorphic state of the SSRs are generally species specific they need to be identified for each species individually.
We have developed a novel strategy that combines a sampling strategy, SSR enrichment and next generation sequencing to simultaneously isolate SSRs and identify those that are polymorphic within the germplasm of interest (patent pending). This strategy facilitates the identification of a useful set of polymorphic SSRs for all plant species independent of the availability of any other molecular information.
Modern molecular techniques have been developed to meet the demands of the agriculture and horticulture industries.
A major bottleneck in the application of molecular techniques to the identification and improvement of plants is the development of the appropriate molecular markers for every species.
The most popular source of markers is the simple sequence repeats (SSRs) found in all eukaryotic genomes.
However, since the primers to characterize the polymorphic state of the SSRs are generally species specific they need to be identified for each species individually.
We have developed a novel strategy that combines a sampling strategy, SSR enrichment and next generation sequencing to simultaneously isolate SSRs and identify those that are polymorphic within the germplasm of interest (patent pending). This strategy facilitates the identification of a useful set of polymorphic SSRs for all plant species independent of the availability of any other molecular information.
Authors
C.A. Cullis, M.A. Cullis, T.A. Cullis
Keywords
SSRs, Tylosema esculentum, marama, FIASCO, 454 sequencing
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