DISCOVERY OF SNP MARKERS IN EXPRESSED GENES OF HAZELNUT

Polymorphisms associated with gene coding regions are useful tools for use as molecular markers, for example in marker-assisted breeding efforts.
In particular, single nucleotide polymorphisms (SNPs) have emerged as a preferred marker for high-throughput genotyping studies.
The objective of this study was to develop a set of SNPs for European hazelnut (Corylus avellana) by exploiting the exceptional depth and breadth of transcriptome sequencing made possible by high-throughput RNA sequencing (RNA-seq) using the Illumina Genome Analyzer platform.
Bioinformatics tools were used to mine SNPs from a database of EST sequences derived from de novo assembly of Illumina RNA-seq reads representing hazelnut transcripts from multiple accessions.
This resulted in the identification of 5,398 SNPs.
This set of SNP markers for hazelnut provides a new resource for genetic mapping of important agronomic traits and will be useful markers for aligning future physical and genetic maps.

International Symposium on Molecular Markers in Horticulture

D.W. Bryant, S.E. Fox, E.R. Rowley, H.D. Priest, R. Shen, W.-K. Wong, T.C. Mockler

Corylus avellana, hazelnut, single nucleotide polymorphism, SNP, RNA-seq, expressed sequence tags, candidate genes, high throughput RNA sequencing

https://doi.org/10.17660/ActaHortic.2010.859.33