Articles
STAYGREEN (SGR), A CANDIDATE GENE FOR THE PERSISTENT COLOR PHENOTYPE IN COMMON BEAN
Article number
859_10
Pages
99 – 102
Language
English
Abstract
The persistent color (pc) trait is valued by snap bean processors for a dark, uniform green pod color.
Cultivars with pc may show reduced germination and emergence in the field.
Understanding the molecular and biochemical basis of pc could suggest breeding strategies to improve field performance while retaining pod quality.
We hypothesized that the gene controlling pc was a member of the staygreen (sgr) family described in rice, garden pea, and Arabidopsis. Using the pea sgr gene as a query, putative soybean and common bean orthologs were identified by BLAST. Primers based on consensus sequences were used to amplify the candidate pc gene from normal and pc common bean lines.
A 1,450 bp band was amplified in normal, but only a 1,000 bp band was amplified in pc lines.
Marker analysis of three populations segregating for the pc locus indicated cosegregation between marker and phenotype, suggesting the pc locus in snap bean is the staygreen gene.
A SNP was discovered in the parents of the BAT96 × Jalo EEP558 mapping population and used to map the locus on linkage group Pv2. Independent mapping studies are under way to determine if the map location cosegregates with the pc phenotype predicted location of pc in common bean.
Cultivars with pc may show reduced germination and emergence in the field.
Understanding the molecular and biochemical basis of pc could suggest breeding strategies to improve field performance while retaining pod quality.
We hypothesized that the gene controlling pc was a member of the staygreen (sgr) family described in rice, garden pea, and Arabidopsis. Using the pea sgr gene as a query, putative soybean and common bean orthologs were identified by BLAST. Primers based on consensus sequences were used to amplify the candidate pc gene from normal and pc common bean lines.
A 1,450 bp band was amplified in normal, but only a 1,000 bp band was amplified in pc lines.
Marker analysis of three populations segregating for the pc locus indicated cosegregation between marker and phenotype, suggesting the pc locus in snap bean is the staygreen gene.
A SNP was discovered in the parents of the BAT96 × Jalo EEP558 mapping population and used to map the locus on linkage group Pv2. Independent mapping studies are under way to determine if the map location cosegregates with the pc phenotype predicted location of pc in common bean.
Authors
J. Davis, J.R. Myers, P. McClean, R. Lee
Keywords
snap bean, senescence, staygreen, Phaseolus vulgaris
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