Articles
REMAP AS A TOOL FOR PRELIMINARY GRAPEVINE ACCESSION SCREENING
Article number
859_18
Pages
155 – 159
Language
English
Abstract
One of the main problems in managing germplasm collections of perennial crops, such as grapevine, is the occurrence of synonyms and homonyms among accessions.
Traditional identification procedures based on morphological characters are not consistent and reliable in such cases, consequently genetic identification using DNA-based markers has become popular.
In particular microsatellite markers (SSR) are commonly used for grapevine genotyping.
Although SSRs are codominant, highly reproducible and informative, they can be rather laborious.
Traditional electrophoresis systems (polyacrylamide gels followed by silver staining) are being used or, in the case of capillary electrophoresis, fluorescently labeled primers but make the analysis per sample costly.
In grapevine, at least six SSR loci should be analyzed for reliable identification.
In this study, a set of Vitis vinifera L. cultivars was chosen and used to test the discriminating power of REtrotransposon-Microsatellite Amplified Polymorphism (REMAP) using Gret1 LTR primers in comparison to a common set of SSR loci.
Each of the tested cultivars could be distinguished by using only one or two primer combinations by agarose-based electrophoresis resulting in a cheap and useful tool for preliminary accession screening.
Traditional identification procedures based on morphological characters are not consistent and reliable in such cases, consequently genetic identification using DNA-based markers has become popular.
In particular microsatellite markers (SSR) are commonly used for grapevine genotyping.
Although SSRs are codominant, highly reproducible and informative, they can be rather laborious.
Traditional electrophoresis systems (polyacrylamide gels followed by silver staining) are being used or, in the case of capillary electrophoresis, fluorescently labeled primers but make the analysis per sample costly.
In grapevine, at least six SSR loci should be analyzed for reliable identification.
In this study, a set of Vitis vinifera L. cultivars was chosen and used to test the discriminating power of REtrotransposon-Microsatellite Amplified Polymorphism (REMAP) using Gret1 LTR primers in comparison to a common set of SSR loci.
Each of the tested cultivars could be distinguished by using only one or two primer combinations by agarose-based electrophoresis resulting in a cheap and useful tool for preliminary accession screening.
Authors
S. Šimon, M. Zulj, D. Preiner, I. Pejić, F. Gaši, N. Malenica, G. Zdunic
Keywords
Vitis, accession screening, SSR, REtrotransposon-Microsatellite Amplified Polymorphism, Gret1 LTR primers
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