Articles
GENETIC DIVERSITY AND TAXON DELINEATION OF ILEX GLABRA USING AFLP MARKERS
Article number
859_30
Pages
261 – 269
Language
English
Abstract
Ilex glabra (Inkberry) is a native evergreen shrub with dark green foliage and compact habit.
This shrub has gained popularity in the northern landscapes of the United States and more nursery growers would like to produce it.
To better understand genetic relationships among inkberry cultivars and selectively breed superior cultivars, a group of 48 Ilex glabra accessions and two other Ilex species (Ilex crenata and I. mutchagara) were studied using AFLP markers.
A total of 229 markers between 50 and 500 base pairs (bps) were produced from eight AFLP primer combinations.
Among them, 87% of markers produced were polymorphic.
The markers for each primer-pair ranged from 22 to 45 and the genetic distance observed ranged from 0.001 to 0.349. Within the Ilex glabra clade, 48 accessions were classified into six groups including a wild species group, Ilex glabra and five cultivated groups, Densa, f. leucocarpa, Pretty Girl, Shamrock and Viridis. Within each group, the legitimacy of named cultivars and distinguished clones are discussed based on both morphological and molecular data.
The AFLP data provided useful information to researchers and growers for inkberry cultivar identification, genetic improvement, and germplasm conservation.
This shrub has gained popularity in the northern landscapes of the United States and more nursery growers would like to produce it.
To better understand genetic relationships among inkberry cultivars and selectively breed superior cultivars, a group of 48 Ilex glabra accessions and two other Ilex species (Ilex crenata and I. mutchagara) were studied using AFLP markers.
A total of 229 markers between 50 and 500 base pairs (bps) were produced from eight AFLP primer combinations.
Among them, 87% of markers produced were polymorphic.
The markers for each primer-pair ranged from 22 to 45 and the genetic distance observed ranged from 0.001 to 0.349. Within the Ilex glabra clade, 48 accessions were classified into six groups including a wild species group, Ilex glabra and five cultivated groups, Densa, f. leucocarpa, Pretty Girl, Shamrock and Viridis. Within each group, the legitimacy of named cultivars and distinguished clones are discussed based on both morphological and molecular data.
The AFLP data provided useful information to researchers and growers for inkberry cultivar identification, genetic improvement, and germplasm conservation.
Authors
Youping Sun, Donglin Zhang, Fang Geng
Keywords
‘Densa’, inkberry, ‘Pretty Girl’, ‘Shamrock’, ‘Viridis’
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