Articles
IDENTIFICATION OF TWO SRAP MARKERS LINKED TO GREEN SKIN COLOR IN CUCUMBER
Article number
859_41
Pages
351 – 356
Language
English
Abstract
Fruit color is a very important trait in cucumber.
In this study, bulked segregant analysis (BSA) was used to identify sequence-related amplified polymorphism (SRAP) markers linked to green skin color.
We used a segregating F2 population consisting of 123 seedlings from a cross between WD3 with green skin and B-2-2 with white skin (WD3×B-2-2) to identify closely-linked markers with fruit color.
Linkage analysis identified two alleles, ME9EM1-300 and ME8EM14-425 associated with green skin color.
These markers were 6.0 and 8.3 cM, respectively, from the skin color locus.
By using these molecular markers, cucumber breeding could be accelerated; breeders could identify and select plants able to produce green skin color fruits at the seedling stage without waiting until the mature fruit stage.
In this study, bulked segregant analysis (BSA) was used to identify sequence-related amplified polymorphism (SRAP) markers linked to green skin color.
We used a segregating F2 population consisting of 123 seedlings from a cross between WD3 with green skin and B-2-2 with white skin (WD3×B-2-2) to identify closely-linked markers with fruit color.
Linkage analysis identified two alleles, ME9EM1-300 and ME8EM14-425 associated with green skin color.
These markers were 6.0 and 8.3 cM, respectively, from the skin color locus.
By using these molecular markers, cucumber breeding could be accelerated; breeders could identify and select plants able to produce green skin color fruits at the seedling stage without waiting until the mature fruit stage.
Authors
Shu-xia Chen, Huan-wen Meng, Zhi-hui Cheng, Ya-li Li, Yangling Shaanxi, Dan-dan Chai
Keywords
fruit color, SRAP marker, linkage mapping, cucumber
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