Articles
WHOLE-GENOME SNP SEQUENCING OF SELECTED PROGENY INDIVIDUALS ALLOWS ANCHORING OF DRAFT GENOME SEQUENCE TO A LINKAGE MAP FOR THE APPLE SCAB PATHOGEN VENTURIA INAEQUALIS
Article number
859_34
Pages
295 – 300
Language
English
Abstract
Determining the position and order of contigs within an organisms genome remains a technical challenge in a majority of sequencing projects.
To exploit contemporary technologies for DNA sequencing, we developed a strategy for whole genome SNP sequencing allowing the positioning of contigs onto a linkage map using the bin mapping method.
We demonstrated the utility of this strategy by accurately determining the joint genotypes of a portion of large contigs and validated our method by mapping SSR markers developed from bin-mapped sequences from a draft genome assembly of the apple scab pathogen, Venturia inaequalis.
To exploit contemporary technologies for DNA sequencing, we developed a strategy for whole genome SNP sequencing allowing the positioning of contigs onto a linkage map using the bin mapping method.
We demonstrated the utility of this strategy by accurately determining the joint genotypes of a portion of large contigs and validated our method by mapping SSR markers developed from bin-mapped sequences from a draft genome assembly of the apple scab pathogen, Venturia inaequalis.
Authors
D.J.G. Rees, J.-M. Celton, A. Cristoffels, D.J. Sargent, X. Xu
Keywords
Venturia inaequalis, sequencing, SNP, bin mapping
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