Articles
FINGERPRINTING PRACTICES APPLIED TO THE KOB HERITAGE APPLE CULTIVARS USING SSRS AS PROPOSED BY THE ECPGR_FN
Article number
859_22
Pages
183 – 190
Language
English
Abstract
Using the newly proposed international standards from the European Cooperative Programme for Plant Genetic Resources Fruit Network (ECPGR_FN) based on 12 SSRs, 249 heritage apple cultivars from the Bodensee region growing in the KOB apple preservation orchard were fingerprinted using a CEQ 8000 Sequencer.
Eight genotypes selected from East Malling Research Station (UK) were included as controls.
One hundred and eighty-three alleles were detected which allowed differentiation of each cultivar.
Eleven unknown cultivars were also identified.
Two unknown cultivars did not match the fingerprints of any known cultivar included in this study.
Future expansion of the gene bank with the addition of additional heritage cultivars will create a resource for breeding, nursery gardens and quality control.
Eight genotypes selected from East Malling Research Station (UK) were included as controls.
One hundred and eighty-three alleles were detected which allowed differentiation of each cultivar.
Eleven unknown cultivars were also identified.
Two unknown cultivars did not match the fingerprints of any known cultivar included in this study.
Future expansion of the gene bank with the addition of additional heritage cultivars will create a resource for breeding, nursery gardens and quality control.
Authors
H. Xuan, U. Mayr, M. Büchele
Keywords
microsatellites, genebank, simple sequence repeats, Malus × domestica
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