Articles
SSRS MOLECULAR CHARACTERIZATION OF CASTANEA SATIVA MILL. GENOTYPES SELECTED FOR RESISTANCE TO PHYTOPHTHORA CINNAMOMI
Article number
866_21
Pages
181 – 187
Language
English
Abstract
The objective of this work is to establish a reliable and reproducible molecular method based on SSRs, which allows us to carry out a molecular characterization of selected Phytophthora cinnamomi resistant trees for their certification.
Molecular analyses were performed on three Asiatic Castanea species used in hybridization programs with C. sativa. Moreover, C. sativa plants including forest provenances, and fruit selections, and four selected P. cinnamomi resistant clones have also been analyzed.
Twelve SSRs loci previously described on C. sativa were assayed.
The results obtained showed that ten of the twelve SSRs loci tested (KT002a, KT015a, CsCAT1, CsCAT3, CsCAT6, CsCAT14, CsCAT16, EMCs14, QpZag36, QpZag110) generated reproducible fragments and were used for genotype identification.
A genetic similarity matrix based of the Dice coefficient was obtained with the microsatellite data.
This matrix was used to group all accessions using UPGMA. The generated dendrogram showed two main clusters well defined and separated, with a low genetic similarity coefficient (0.09). Cluster number I was subdivided in two subclusters grouping all C. crenata and C. sativa plants respectively.
All selected P. cinnamomi resistant clones grouped with the C. sativa accessions, except one of the selected clones from A Coruña.
Cluster number II was also subdivided in two subclusters, grouping all C. mollissima and C. henryi plants respectively.
Microsatellite analysis allowed us to assign with some reliability the Phytophthora resistant trees to a given species.
Nevertheless, this reliability will be improved by increasing the genetic variability to analyze, which is a work that is currently in progress.
Molecular analyses were performed on three Asiatic Castanea species used in hybridization programs with C. sativa. Moreover, C. sativa plants including forest provenances, and fruit selections, and four selected P. cinnamomi resistant clones have also been analyzed.
Twelve SSRs loci previously described on C. sativa were assayed.
The results obtained showed that ten of the twelve SSRs loci tested (KT002a, KT015a, CsCAT1, CsCAT3, CsCAT6, CsCAT14, CsCAT16, EMCs14, QpZag36, QpZag110) generated reproducible fragments and were used for genotype identification.
A genetic similarity matrix based of the Dice coefficient was obtained with the microsatellite data.
This matrix was used to group all accessions using UPGMA. The generated dendrogram showed two main clusters well defined and separated, with a low genetic similarity coefficient (0.09). Cluster number I was subdivided in two subclusters grouping all C. crenata and C. sativa plants respectively.
All selected P. cinnamomi resistant clones grouped with the C. sativa accessions, except one of the selected clones from A Coruña.
Cluster number II was also subdivided in two subclusters, grouping all C. mollissima and C. henryi plants respectively.
Microsatellite analysis allowed us to assign with some reliability the Phytophthora resistant trees to a given species.
Nevertheless, this reliability will be improved by increasing the genetic variability to analyze, which is a work that is currently in progress.
Publication
Authors
B. Cuenca, M. Rey, M.V. González, M. López
Keywords
chestnut, microsatellite, field selection, molecular markers, ink disease resistance
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