Home > I European Congress on Chestnut – Castanea 2009 > USE OF MRNA AS AN INDICATOR OF THE VIABILITY OF PHYTOPHTHORA CAMBIVORA

Articles

USE OF MRNA AS AN INDICATOR OF THE VIABILITY OF PHYTOPHTHORA CAMBIVORA

Article number

866_57

Pages

431 – 434

Language

English

Abstract

The assessment of the risk of Ink disease and the evaluation of the efficacy of control measures need a quantitative and qualitative monitoring of Phytophthora cambivora inoculum in soil and host tissues.
Diagnostic methods based on isolation on pure culture are time consuming and poorly sensible.
However DNA based detection methods do not provide information on viability of the inoculum.
Viability in biological samples can be evaluated using RNA transcripts rapidly degraded in dead cells.
In this study the possibility to utilise mRNA transcripts of cytochrome oxidase II gene (Cox II) as marker of viability of Phytophthora cambivora was investigated in vitro following treatment with potential physical and chemical inductors.
Cox II transcripts are detectable in Real-Time RT-PCR and their expression increased up to 6 times following treatment with glucose, KNO₃ and K₃PO₃.

Publication

I European Congress on Chestnut – Castanea 2009

Authors

A.M. Vettraino, A. Tomassini, A. Vannini

Keywords

Ink Disease, chestnut, cytochrome oxidase, inoculum, treatments, induction, real time RT-PCR

Full text

https://doi.org/10.17660/ActaHortic.2010.866.57

Online Articles (95)

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2. THE HISTORICAL ECOLOGY OF LIGURIAN CHESTNUT GROVES: ARCHIVAL DOCUMENTATION AND FIELD EVIDENCE

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5. EPIPHYTIC LICHEN DIVERSITY IN CHESTNUT WOODS

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6. THE POTENTIAL FOR CHESTNUT IN LEBANON: ON THE DESERT EDGE!

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F. Villani | C. Mattioni | M. Cherubini | M. Lauteri | M.A. Martin

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14. SOME PHENOLOGICAL PROPERTIES OF CHESTNUT GENOTYPES IN THE BLACK SEA REGION, TURKEY

U. Serdar | N. Beyhan | L. Demirsoy | H. Demirsoy

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C. Mattioni | M. Cherubini | D. Taurchini | F. Villani | M.A. Martin

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M.A. Martin | C. Mattioni | M. Cherubini | D. Taurchini | F. Villani

18. GENETIC DIVERSITY IN EUROPEAN CHESTNUT POPULATIONS

C. Mattioni | M. Cherubini | D. Taurchini | F. Villani | M.A. Martin

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B. Cuenca | M. Rey | M.V. González | M. López

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L.-T.J. Dinis | F. Peixoto | R. Costa | J. Gomes-Laranjo

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L.L. Cheng | W.G. Huang

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B.M. Lushaj | V. Tabaku

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J. Gomes-Laranjo | E.J. Gonçalo | F. Peixoto

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57. USE OF MRNA AS AN INDICATOR OF THE VIABILITY OF PHYTOPHTHORA CAMBIVORA

A.M. Vettraino | A. Tomassini | A. Vannini

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M. Botu

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S. Bratanova-Doncheva | N. Chipev | V. Dimitrova

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D.W. Fulbright | M. Mandujano | S. Stadt

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M.V. Pridnya | M.D. Pinkovsky | A.V. Romashin

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B.M. Lushaj | V. Tabaku

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I.R. Donis González | D.W. Fulbright | E.T. Ryser | D. Guyer

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V. Uylaser | B.K. Incedayı | C. Mert | A. Soylu

78. POTENTIALITIES OF CHESTNUT FOR STARCH PRODUCTION

P. Correia | M.L. Beirão-da-Costa

79. THERMAL PROCESSES IN THE CANDY PROCESS OF CHESTNUT

L. Comba | P. Gay | P. Piccarolo | D. Ricauda Aimonino

80. INFLUENCE OF SELECTED FACTORS ON EFFICIENCY AND EFFECTIVENESS OF A PEELING MACHINE FOR CHESTNUT

D. Guyer | J. Xing | D.W. Fulbright | M. Mandujano

81. IMPACTS OF MECHANIZED WOOD HARVESTING IN CHESTNUT COPPICES: A CASE STUDY

S. Baldini | F. Di Fulvio | D. Liberati

82. INDUSTRIAL PROCESSING OF CHESTNUT FRUITS (CASTANEA SATIVA MILL.) – EFFECTS ON NUTRIENTS AND PHYTOCHEMICALS

M.C. De Vasconcelos | R. Bennett | E. Rosa | J.V. Ferreira-Cardoso | F. Nunes

83. CHARACTERIZATION OF BAKING PROPERTIES OF CHESTNUT DOUGH

F. Chenlo | R. Moreira | M.D. Torres

84. THEORETICAL DETERMINATION OF SORPTION ISOTHERMS APPLIED TO PROCESS CHESTNUT WITH OSMOTIC PRE-TREATMENTS BASED ON ITS CHEMICAL COMPOSITION

F. Chenlo | R. Moreira | M.D. Torres

85. INFLUENCE OF DRYING TEMPERATURE ON DRYING KINETICS AND PHYSICO-CHEMICAL PROPERTIES OF TWO CHESTNUT VARIETIES (CASTAENEA SATIVA MILL.)

86. CAPACITY OF EXTRACTS OF CASTANEA SATIVA MILL. FOR REMOVAL OF ORGANIC, HYDROPHILIC RADICALS

87. EVALUATION OF THE EFFECTS OF ROASTING ON CHESTNUT FLOUR

N. Andreoni

88. CHESTNUTS, A “COMFORT” HEALTHY FOOD?

89. PROXIMATE CONTENT AND STARCH GRANULE STRUCTURE IN RAW AND BOILED CHESTNUTS WITH DIFFERENT APTITUDE TO CANDYING

C. Mert

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M. Lauteri | M.C. Monteverdi | G. Scarascia-Mugnozza

91. QUALITY WOOD PRODUCTION FROM CHESTNUT (CASTANEA SATIVA MILL.) COPPICE FORESTS – COMPARISON BETWEEN DIFFERENT SILVICULTURAL APPROACHES

92. PROBLEMS AND PROSPECTS OF THE CHESTNUT TIMBER CHAIN IN TUSCANY

C. Becagli | E. Amorini | R. Fratini | M.C. Manetti | E. Marone

93. RESULTS OF EXPERIMENTS ON CHESTNUT CULTIVARS FOR WOOD PRODUCTION

A. Tani | A. Maltoni | B. Mariotti

94. ABOVEGROUND PRODUCTION AND C FLUX IN THREE CHESTNUT HIGH FOREST STANDS IN NORTHERN PORTUGAL

M.S. Patrício | M.J. Fernandes | E. Pereira | D. Lopes

95. GROWTH AND BIOMASS OF TWO-YEAR-OLD SEEDLINGS OF EUROPEAN CHESTNUT DERIVED FROM THE HALF-SIB FAMILIES OF DIFFERENT ORIGIN

M. Bolvanský | D. Tarinová | M. Užík