Articles

DETERMINATION OF HYPOVIRULENT ISOLATES OF CRYPHONECTRIA PARASITICA BY DSRNA ANALYSIS IN AYDIN PROVINCE, TURKEY

Article number
866_49
Pages
379 – 383
Language
English
Abstract
Cryphonectria parasitica (Murr.) Barr isolates which were obtained from the major chestnut growing areas of Aydın, Turkey and selected as hypovirulent according to the canker formation and their cultural characteristics were assayed for the presence of dsRNA. DsRNA was isolated from the mycelia of these isolates which were grown in liquid culture.
After purification, the dsRNA’s were analyzed by agarose gel electrophoresis.
Among the isolates only one was found to contain dsRNA. It was observed that dsRNA banding patterns isolated from the single hypovirulent isolate and from known hypovirulent strains from the USA are similar in number and molecular size which is between 12.0 and 13.0 kbp dsRNA segment.

Publication
Authors
S. Açıkgöz, M.T. Döken, O. Erincik, F. Değirmenci
Keywords
chestnut blight, hypovirulent strains
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