Articles
MEMBRANE LIPID COMPOSITION IN BRASSICA OLERACEA PLANTS WITH DIFFERENT RESISTANCE TO DOWNY MILDEW
Article number
917_20
Pages
159 – 166
Language
English
Abstract
This work was a preliminary study focusing on membrane lipid composition of leaves of Brassica oleracea var. italica (broccoli) and var. tronchuda (tronchuda cabbage) populations differing in adult plant downy mildew (DM) resistance.
Plants with different DM response were selected in a field experiment with F3 families from a cross between a susceptible rapid-cycling brassica and two resistant lines (broccoli and Couve Algarvia). Leaf samples from the two parental brassica resistant lines (R), and six F3 resistant (F3R) and susceptible (F3S) families from each cross were collected for total lipids extraction.
Fatty acids were analysed by gas chromatography.
The fatty acids profiles of R, F3R, and F3S were different in broccoli and Couve Algarvia. In broccoli, higher C18:3 values were observed in R and F3R plants when compared to F3S plants The same occurred for C16:3 although it was not significant for R plants.
Higher lipids unsaturation (DBI) was observed in F3R broccoli families as a result of trienoic acids increases (C18:3 and C16:3) and concomitant C16:0 lowering.
In Couve Algarvia, F3S showed higher amounts of C16:3 and a lower C16:1 value than the other plants.
DBI values of R and F3R plants were lower than F3S. No clear differences were observed in leaf lipid composition between DM resistant and susceptible broccoli and Couve Algarvia plants.
Pattern variation and differences in lipid profile between genotypes need to be further investigated using a larger number of plants and genotypes.
This study also highlighted an influence of low temperature on membrane lipid composition of broccoli, suggesting future experiments should be performed under controlled conditions.
Plants with different DM response were selected in a field experiment with F3 families from a cross between a susceptible rapid-cycling brassica and two resistant lines (broccoli and Couve Algarvia). Leaf samples from the two parental brassica resistant lines (R), and six F3 resistant (F3R) and susceptible (F3S) families from each cross were collected for total lipids extraction.
Fatty acids were analysed by gas chromatography.
The fatty acids profiles of R, F3R, and F3S were different in broccoli and Couve Algarvia. In broccoli, higher C18:3 values were observed in R and F3R plants when compared to F3S plants The same occurred for C16:3 although it was not significant for R plants.
Higher lipids unsaturation (DBI) was observed in F3R broccoli families as a result of trienoic acids increases (C18:3 and C16:3) and concomitant C16:0 lowering.
In Couve Algarvia, F3S showed higher amounts of C16:3 and a lower C16:1 value than the other plants.
DBI values of R and F3R plants were lower than F3S. No clear differences were observed in leaf lipid composition between DM resistant and susceptible broccoli and Couve Algarvia plants.
Pattern variation and differences in lipid profile between genotypes need to be further investigated using a larger number of plants and genotypes.
This study also highlighted an influence of low temperature on membrane lipid composition of broccoli, suggesting future experiments should be performed under controlled conditions.
Authors
P. Scotti Campos, I.P. Pais, J.N. Semedo, P.R. Ramos, C.S. Santiago , P.S. Coelho, L.P. Valério , A.A. Monteiro
Keywords
Hyaloperonospora parasitica, field resistance, oomycete, fatty acids unsaturation
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