Articles
DETECTION, OCCURRENCE AND NATURAL INCIDENCE OF PEPPER MILD MOTTLE VIRUS (PMMOV) IN HOT PEPPERS IN BRAZIL
Article number
917_37
Pages
269 – 273
Language
English
Abstract
There are several viral diseases that can cause economic losses to hot peppers (Capsicum spp.). Among these viruses, Pepper mild mottle virus (PMMoV) within Tobamovirus genus, is a serious threat to hot pepper production, fruit quality, and especially to seed production and commercialization, due to its seed-borne trans¬mission ability.
In Brazil, PMMoV was found in 2001 in hot pepper plants under protected cultivation.
In order to detect and evaluate the natural incidence of PMMoV in hot peppers in Brazil, a survey was carried out in fields cultivated with accessions of Capsicum chinense, C. frutescens and C. baccatum var. praetermissum collected from different Brazilian geographical regions.
Plants grown from seeds were transferred to the field 30-45 days after sowing.
Leaf sampling for PMMoV evaluation was performed either just before or soon after flowering from a total of 482 plants.
Sample analysis was done by enzyme-linked immunosorbent assay (ELISA), using a polyclonal antiserum raised against the coat protein of PMMoV, in addition to symptom evaluation on field-grown plants.
PMMoV detection by ELISA was confirmed in 217/482 samples with or without disease symptoms.
These data indicate an increasing incidence of PMMoV in hot peppers in the country since the first detection in 2001, and also that the virus is widespread in this crop in different geographical regions in Brazil.
This information supports the urgent need for the development of efficient and effective disease management strategies, considering the high stability of the virus particles that can be easily transmitted to healthy plants by workers hands and contact between plants, together with adequate legislation.
Furthermore, preliminary evaluation of distinct seed treatment methods showed a low efficiency, demonstrating that a comprehensive survey on PMMoV incidence and control must be implemented in Brazil.
In Brazil, PMMoV was found in 2001 in hot pepper plants under protected cultivation.
In order to detect and evaluate the natural incidence of PMMoV in hot peppers in Brazil, a survey was carried out in fields cultivated with accessions of Capsicum chinense, C. frutescens and C. baccatum var. praetermissum collected from different Brazilian geographical regions.
Plants grown from seeds were transferred to the field 30-45 days after sowing.
Leaf sampling for PMMoV evaluation was performed either just before or soon after flowering from a total of 482 plants.
Sample analysis was done by enzyme-linked immunosorbent assay (ELISA), using a polyclonal antiserum raised against the coat protein of PMMoV, in addition to symptom evaluation on field-grown plants.
PMMoV detection by ELISA was confirmed in 217/482 samples with or without disease symptoms.
These data indicate an increasing incidence of PMMoV in hot peppers in the country since the first detection in 2001, and also that the virus is widespread in this crop in different geographical regions in Brazil.
This information supports the urgent need for the development of efficient and effective disease management strategies, considering the high stability of the virus particles that can be easily transmitted to healthy plants by workers hands and contact between plants, together with adequate legislation.
Furthermore, preliminary evaluation of distinct seed treatment methods showed a low efficiency, demonstrating that a comprehensive survey on PMMoV incidence and control must be implemented in Brazil.
Authors
M.F. Lima, A.K. Inoue-Nagata , F.J.B. Reifschneider, K.R.R. Souza , A.B. Ulhoa, R.M. Ferraz
Keywords
pepper, PMMoV survey, seed-borne, Capsicum spp., incidence
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