Articles
VIRUS INCIDENCE IN DOMESTICATED AND SEMI-DOMESTICATED FIELD-GROWN HOT PEPPERS (CAPSICUM SPP.)
Article number
917_40
Pages
285 – 290
Language
English
Abstract
Viruses are the main pathogens of hot peppers (Capsicum spp.) in Brazil, directly affecting yield and marketable fruit quality.
Disease incidence related to natural infection by viruses was assessed in domesticated and semi-domesticated Malagueta (Capsicum frutescens), Habanero (Capsicum chinense) and Cumari (Capsicum baccatum var. praetermissum) hot pepper accessions from Embrapa Vegetables Active Germplasm Bank, Brasília, Brazil.
Seedlings were grown from treated seeds in the greenhouse and transferred to the field 30-45 days after sowing; trials were located in the vicinity of a naturally virus-infected pepper field.
Sampling was performed after 60 days post transplanting when plants were 90-105 days old.
A total of 482 plants was individually sampled, and tested for the presence of tospo¬viruses (Tomato spotted wilt virus – TSWV; Groundnut ringspot virus – GRSV; Tomato chlorotic spot virus – TCSV), potyviruses (Potato virus Y – PVY; Pepper yellow mosaic virus – PepYMV), and tobamovirus (Pepper mild mottle virus – PMMoV) by enzyme-linked immunosorbent assay (DAS-ELISA), and for cucumovirus (Cucumber mosaic virus – CMV) by dot-ELISA. Based on results of serological tests, 67% out of 482 plants presented single or mixed infections.
The proportion of positive samples ranged from 9.1% (TCSV; 44 plants) to 45% (PMMoV; 217). PVY, PepYMV, TSWV and GRSV were found in 34.2% (165 samples), 25.5% (123), 15.7% (75) and 13.3% (64) of the tested-samples, respectively.
CMV was not detected.
It is noteworthy that virus infection was also detected on plants of the semi-domesticated genotype Cumari. These results indicate the importance of viral diseases to crop management, con¬sidering the lack of efficient virus management strategies and the scarce information available on virus incidence affecting the crop, as well.
A better understanding of the epidemiology of viruses infecting hot peppers will contribute to the development of rational disease management to prevent virus outbreaks.
Disease incidence related to natural infection by viruses was assessed in domesticated and semi-domesticated Malagueta (Capsicum frutescens), Habanero (Capsicum chinense) and Cumari (Capsicum baccatum var. praetermissum) hot pepper accessions from Embrapa Vegetables Active Germplasm Bank, Brasília, Brazil.
Seedlings were grown from treated seeds in the greenhouse and transferred to the field 30-45 days after sowing; trials were located in the vicinity of a naturally virus-infected pepper field.
Sampling was performed after 60 days post transplanting when plants were 90-105 days old.
A total of 482 plants was individually sampled, and tested for the presence of tospo¬viruses (Tomato spotted wilt virus – TSWV; Groundnut ringspot virus – GRSV; Tomato chlorotic spot virus – TCSV), potyviruses (Potato virus Y – PVY; Pepper yellow mosaic virus – PepYMV), and tobamovirus (Pepper mild mottle virus – PMMoV) by enzyme-linked immunosorbent assay (DAS-ELISA), and for cucumovirus (Cucumber mosaic virus – CMV) by dot-ELISA. Based on results of serological tests, 67% out of 482 plants presented single or mixed infections.
The proportion of positive samples ranged from 9.1% (TCSV; 44 plants) to 45% (PMMoV; 217). PVY, PepYMV, TSWV and GRSV were found in 34.2% (165 samples), 25.5% (123), 15.7% (75) and 13.3% (64) of the tested-samples, respectively.
CMV was not detected.
It is noteworthy that virus infection was also detected on plants of the semi-domesticated genotype Cumari. These results indicate the importance of viral diseases to crop management, con¬sidering the lack of efficient virus management strategies and the scarce information available on virus incidence affecting the crop, as well.
A better understanding of the epidemiology of viruses infecting hot peppers will contribute to the development of rational disease management to prevent virus outbreaks.
Authors
M.F. Lima, A.K. Inoue-Nagata , F.J.B. Reifschneider, A.B. Ulhoa , K.R.R. Souza, R.M. Ferraz
Keywords
virus incidence, virus detection, Tomato spotted wilt virus, Groundnut ringspot virus, Potato virus Y, Pepper yellow mosaic virus
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