Articles
OLIVE MOLECULAR RESPONSE TO ATTACK OF BACTROCERA OLEAE: IDENTIFICATION OF UP-REGULATED GENES IN INFESTED OLIVE FRUITS
Article number
929_17
Pages
125 – 128
Language
English
Abstract
The olive fruit fly Bactrocera oleae (Rossi) is an important pest of olive fruits, that causes a significant reduction of fruit yield and quality.
In order to study the molecular response of olive fruits to olive fly attack and to identify genes involved in the olive defence response, we have constructed a SSH (Suppression Subtractive Hybridisation) cDNA library, from RNA isolated from Moraiolo. Clones showing an insert size greater than 200 bp (219) were sequenced and functionally annotated.
Only 48% of the ESTs could be classified into putative known functions.
The response of olive fruits to B. oleae infestation proved to be complex and involving numerous molecular functions.
The differential expression of the most interesting transcripts was analyzed by RT-PCR. The obtained results confirmed the overexpression of defense related sequences in the olive tissue infested by the fruit fly.
This study represents an initial step toward the understanding of the molecular basis of the interaction between olive fruits and B. oleae.
In order to study the molecular response of olive fruits to olive fly attack and to identify genes involved in the olive defence response, we have constructed a SSH (Suppression Subtractive Hybridisation) cDNA library, from RNA isolated from Moraiolo. Clones showing an insert size greater than 200 bp (219) were sequenced and functionally annotated.
Only 48% of the ESTs could be classified into putative known functions.
The response of olive fruits to B. oleae infestation proved to be complex and involving numerous molecular functions.
The differential expression of the most interesting transcripts was analyzed by RT-PCR. The obtained results confirmed the overexpression of defense related sequences in the olive tissue infested by the fruit fly.
This study represents an initial step toward the understanding of the molecular basis of the interaction between olive fruits and B. oleae.
Authors
A. Imperato, G. Corrado, F. Alagna, P. Varricchio, L. Baldoni, R. Rao
Keywords
Olea europea, SSH-PCR Select, expressed sequence tags
Online Articles (66)