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USE OF SCAR PCR IN SEX DETERMINATION OF TWO WILD PISTACIA SEEDLINGS

Article number
929_36
Pages
253 – 254
Language
English
Abstract
Pistacia atlantica Desf. mutica and Pistacia khinjuk are dioecious plants.
In addition to having a high nutritional and medical value, they are important Pistacia vera rootstocks.
Quick gender identification in juvenile stage would result in great saving in time and cost.
With respect to the long juvenile stage of Pistacia species, marker assistance selection can facilitated breeding, Sequence Characterized Amplified Region (SCAR) was used to determine sex in these two wild species.
According to the results, a 297 bp fragment was amplified in all female trees which was absent in male trees.
Although sex determination in Pistacia is still unknown, correlations between the SCAR marker and sex locus in Pistacia species, sex determination in Pistacia species is possibly controlled by a single or a few loci in this genus.
Consequently by using SCAR PCR technique, it is possible to detect sexual phenotype of Pistacia atlantica Desf. mutica and Pistacia khinjuk at the early juvenile stage.

Publication
XXVIII International Horticultural Congress on Science and Horticulture for People (IHC2010): International Symposium on Genomics and Genetic Transformation of Horticultural Crops
Authors
B. Esfandiyari, G.H. Davarynejad, F.A. Shahriyari , M. Kiani
Keywords
wild Pistacia, sex determination, SCAR PCR
Full text
https://doi.org/10.17660/ActaHortic.2012.929.36
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