Articles
DISTINCTIVE TRANSCRIPTOME RESPONSE OF TWO STRAWBERRY (FRAGARIA × ANANASSA) CULTIVARS TO COLLETOTRICHUM ACUTATUM INFECTION
Article number
929_5
Pages
47 – 50
Language
English
Abstract
Strawberry (Fragaria × ananassa) yields are strongly affected by fungal diseases and pests, e.g., Colletotrichum spp., forcing the excessive use of chemical products (mostly environmental contaminants) to control them.
Resistant cultivars are a priority of most strawberry breeding programs; however, little is known about the genetic basis for strawberrys resistance to pathogens, and completely resistant cultivars have not been reported.
Although molecular markers of disease resistance in strawberry have been reported, the octoploid genetic structure of commercial strawberry makes it difficult to associate molecular markers with disease resistance genes.
To gain insights into the genetic mechanisms of defense against Colletotrichum acutatum, the transcriptome of two strawberry cultivars (Camarosa and Andana) exhibiting different susceptibility to this pathogen has been analyzed.
An in-house cDNA microarray based on a 3264 strawberry probe set was fabricated from a strawberry differential ESTs collection previously obtained in response to this pathogen.
Transcriptional comparison of these two cultivars was performed before and after infection.
The expression of 190 genes was significantly altered, of which, 94 and 50 genes, were exclusively alterated in cultivars Camarosa and Andana, respectively.
Over-expression and siRNA approaches are being used for functional analysis of candidate genes.
Valuable information is being generated as a tool for effective control strategies to increase resistance in strawberry.
Resistant cultivars are a priority of most strawberry breeding programs; however, little is known about the genetic basis for strawberrys resistance to pathogens, and completely resistant cultivars have not been reported.
Although molecular markers of disease resistance in strawberry have been reported, the octoploid genetic structure of commercial strawberry makes it difficult to associate molecular markers with disease resistance genes.
To gain insights into the genetic mechanisms of defense against Colletotrichum acutatum, the transcriptome of two strawberry cultivars (Camarosa and Andana) exhibiting different susceptibility to this pathogen has been analyzed.
An in-house cDNA microarray based on a 3264 strawberry probe set was fabricated from a strawberry differential ESTs collection previously obtained in response to this pathogen.
Transcriptional comparison of these two cultivars was performed before and after infection.
The expression of 190 genes was significantly altered, of which, 94 and 50 genes, were exclusively alterated in cultivars Camarosa and Andana, respectively.
Over-expression and siRNA approaches are being used for functional analysis of candidate genes.
Valuable information is being generated as a tool for effective control strategies to increase resistance in strawberry.
Authors
F. Amil-Ruiz, S. Encinas-Villarejo, J. Muñoz-Blanco , J.L. Caballero, A. Muñoz-Mérida , O. Trelles, B. de los Santos , F. Romero, J.A. Mercado , F. Pliego-Alfaro
Keywords
anthracnose, strawberry defense, microarray
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