Articles
CONSTRUCTION OF TWO HIGH DENSITY GENETIC LINKAGE MAPS IN LITCHI
Article number
929_29
Pages
207 – 213
Language
English
Abstract
RAPD, AFLP and SRAP techniques were used to construct high density genetic maps of litchi based on the mapping population (83 individuals) derived from a cross of Maguili×Jiaohesanyuehong. The number of segregated markers from the population amounted to 1161, among which the maternal specific markers were 516, paternal specific markers were 438, and the remaining 207 makers were detected in both parents.
Two linkage maps of Maguili and Jiaohesanyuehong were separately constructed with a log-of-odds (LOD) value of 3.5 by JoinMap® 3.0 software.
The linkage map of Maguili consisted of 297 loci in 18 linkage groups, spanned a total map distance of 990.3 cM with an average internal distance of 3.3 cM and the length of the linkages ranged from 21.8 to 130.5 cM. Correspondingly, the genetic linkage map of Jiaohesanyuehong was comprised of 288 loci, which fell into 16 linkage groups with an average internal distance of 2.8 cM, covering a total map distance of 811.9 cM. The linkage length ranged from 4.0 to 135 cM. Taking together, two high densities genetic linkage maps of litchi were constructed, which laid a good foundation for further QTL studies on juvenile phase, maturity period, fruit qualities, etc.
Two linkage maps of Maguili and Jiaohesanyuehong were separately constructed with a log-of-odds (LOD) value of 3.5 by JoinMap® 3.0 software.
The linkage map of Maguili consisted of 297 loci in 18 linkage groups, spanned a total map distance of 990.3 cM with an average internal distance of 3.3 cM and the length of the linkages ranged from 21.8 to 130.5 cM. Correspondingly, the genetic linkage map of Jiaohesanyuehong was comprised of 288 loci, which fell into 16 linkage groups with an average internal distance of 2.8 cM, covering a total map distance of 811.9 cM. The linkage length ranged from 4.0 to 135 cM. Taking together, two high densities genetic linkage maps of litchi were constructed, which laid a good foundation for further QTL studies on juvenile phase, maturity period, fruit qualities, etc.
Authors
J. Zhou, J.-X. Fu, Z.-X. Wu, S.-S. Huang, Y.-H. Zhao, B. Zhang, Y.-L. Hu, G.-B. Hu, C.-M. Liu
Keywords
molecular marker, genetic linkage map, high density
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