TRANSCRIPTOMIC ANALYSIS OF THE RESPONSE OF ‘CARDINAL’ TABLE GRAPES TO LOW TEMPERATURE AND HIGH CO₂

Postharvest storage life of table grapes is limited due to their sensitivity to fungal attack.
A 3-day high CO₂ pre-treatment was effective for maintaining the quality of ‘Cardinal’ berries, but little is known about its mode of action at molecular levels.
To gain a better understanding of the molecular mechanisms involved in the responses of grapes to low temperatures and high CO₂ levels we have performed genome-wide transcriptional profiling analysis of RNA isolated from the skin of ‘Cardinal’ berries treated and non-treated with high CO₂ levels using a custom-made Affymetrix GeneChip; it includes probes for 23,096 unigenes developed within the GrapeGen project.
Results showed that 3 days of pre-treatment with high CO₂ caused higher changes in gene expression compared to non-treated fruit.
By contrast, major changes in gene expression occurred at the end of the storage being higher after 27 days in air (1674), while in CO₂-treated grapes the number of differentially expressed genes was significantly reduced (643).

XXVIII International Horticultural Congress on Science and Horticulture for People (IHC2010): International Symposium on Genomics and Genetic Transformation of Horticultural Crops

C. Fernandez-Caballero, M.I. Escribano, C. Merodio , M.T. Sanchez-Ballesta

Vitis vinifera, carbon dioxide, microarrays, low temperature, transcriptome

https://doi.org/10.17660/ActaHortic.2012.929.32