Articles
HIGH EFFICIENCY TRANSFORMATION PLATFORMS FOR KIWIFRUIT (ACTINIDIA SPP.) FUNCTIONAL GENOMICS
Article number
929_20
Pages
143 – 148
Language
English
Abstract
Agrobacterium-mediated transformation is important in plant functional genomics.
We have established high-throughput transformation platforms for exploiting functional genomics in four Actinidia species (A. arguta, A. chinensis, A. deliciosa and A. eriantha). Leaf explants of kiwifruit (Actinidia spp.) were co-cultivated with Agrobacterium tumefaciens EHA105, harbouring a pART27-derived vector containing the gene of interest under the transcriptional control of the CaMV 35S promoter and the nptII gene as a selection marker.
Transgenic shoots developed approximately 8-16 weeks after explants were transferred to MS medium supplemented with cytokinins, auxins, Timentin® and kanamycin sulfate.
With these efficient transformation platforms, Actinidia spp. has been transformed with >150 kiwifruit cDNAs encoding genes of interest from our Actinidia EST database and >2000 transgenic plants have been produced.
These genes of interest target a broad spectrum of kiwifruit traits including dormancy, budbreak and flowering, vine architecture, fruit development, appearance, flavour and metabolites.
We have established high-throughput transformation platforms for exploiting functional genomics in four Actinidia species (A. arguta, A. chinensis, A. deliciosa and A. eriantha). Leaf explants of kiwifruit (Actinidia spp.) were co-cultivated with Agrobacterium tumefaciens EHA105, harbouring a pART27-derived vector containing the gene of interest under the transcriptional control of the CaMV 35S promoter and the nptII gene as a selection marker.
Transgenic shoots developed approximately 8-16 weeks after explants were transferred to MS medium supplemented with cytokinins, auxins, Timentin® and kanamycin sulfate.
With these efficient transformation platforms, Actinidia spp. has been transformed with >150 kiwifruit cDNAs encoding genes of interest from our Actinidia EST database and >2000 transgenic plants have been produced.
These genes of interest target a broad spectrum of kiwifruit traits including dormancy, budbreak and flowering, vine architecture, fruit development, appearance, flavour and metabolites.
Authors
T. Wang, S. Karunairetnam, R. Wu, Y.-Y. Wang , A.P. Gleave
Keywords
Agrobacterium-mediated transformation, transgenic
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