Become a member

Articles

TRANSGENIC PLUMS (PRUNUS DOMESTICA L.) OF ‘STARTOVAYA’ EXPRESS THE PLUM POX VIRUS COAT PROTEIN GENE

Article number
929_64
Pages
445 – 450
Language
English
Abstract
Plum (P. domestica L.) leaves of ‘Startovaya’ were transformed using the Agrobacterium tumefaciens strain AGL0: pCamPPVcp carrying the hygromycin phosphotransferase gene (hpt) and the coat protein (CP) gene of Plum pox virus (PPV) in the sense orientation.
Leaf explants were co-cultivated with A. tumefaciens, and selection and regeneration of transformed cells and shoots was carried out during 6 months on selection medium supplemented with 5 mg/L hygromycin.
Integration and expression of the transferred genes into regenerated plum plants were verified through a hygromycin resistance and PCR amplification of the PPV-CP gene.
Seven independent transgenic lines were produced from 524 leaf explants.
The accumulation of coat protein was evaluated by Western blot assay.

Publication
XXVIII International Horticultural Congress on Science and Horticulture for People (IHC2010): International Symposium on Genomics and Genetic Transformation of Horticultural Crops
Authors
R. Mikhailov, A. Firsov, O. Shulga, S. Dolgov
Keywords
transformation, somatic tissue, Prunus domestica, PPV
Full text
https://doi.org/10.17660/ActaHortic.2012.929.64
Online Articles (66)
X. Liu | G.L. Reighard | G.A. Swire-Clark | W.C. Bridges | A.G. Abbott | W.V. Baird
K. Wang | Y. Sha | Y. Gong | L. Xu | L. Liu | X. Zhu | D. Huang
C. Leida | J. Terol | G. Llácer | M. Badenes | G. Ríos | G. Martí | M. Agustí
H. Li | Z. Zhang | F. Huang | L. Chang | Y. Ma | X. Zhao
F.R. Tadeo | J. Agustí | P. Merelo | M. Cercós | J. Terol | C. Domingo | M. Talón
F. Amil-Ruiz | S. Encinas-Villarejo | J. Muñoz-Blanco | J.L. Caballero | A. Muñoz-Mérida | O. Trelles | B. de los Santos | F. Romero | J.A. Mercado | F. Pliego-Alfaro
J. He | F. Chen | S. Chen | Z. Guan | W. Fang | Z. Liu | J. Lu
V.J. Febres | A. Khalaf | F.G. Gmitter | G.A. Moore
B. Guitton | V. Segura | J.J. Kelner | T. Foster | S. Gardiner | D. Chagné | E. Costes
G. Koehler | S.K. Randall | R.C. Wilson | P. Winge | J. Rohloff | M. Alsheikh
J. Palmer | L. Lozano | D. Chagné | R. Volz | K. Lin-Wang | J. Bonany | D. Micheletti | M. Troggio | A. White | S. Kumar | A.C. Allan | I. Iglesias
F.F. Rosas-Cárdenas | O. Paredes-López | A. Cruz-Hernández
F. Laurens | M.J. Aranzana | P. Arús | D. Bassi | J. Bonany | L. Corelli | C.E. Durel | J. Mes | T. Pascal | A. Patocchi | A. Peil | B. Quilot | S. Salvi | S. Tartarini | M. Troggio | A. Vecchietti | R. Velasco | E. van de Weg
K.M. Blaker | J.X. Chaparro | T.G. Beckman
J.A. García-Gago | M. Barceló | J.M. López-Aranda | J. Muñoz-Blanco | S. Posé | F. Pliego-Alfaro | J.A. Mercado | M.A. Quesada
T. Borchert | A. Behrend | A. Hohe
A. Fernández i Martí | W. Howad | R. Tao | J.M. Alonso | P. Arús | R. Socias i Company
A. Imperato | G. Corrado | F. Alagna | P. Varricchio | L. Baldoni | R. Rao
Yuelin Zhu | Lifei Yang | Junyi Gai
R. Paris | G. Pagliarani | S. Tartarini | S. Sansavini | C. Gessler | E. van de Weg
T. Wang | S. Karunairetnam | R. Wu | Y.-Y. Wang | A.P. Gleave
S.A. Mahfouze | KH.A. El-Dougdoug
J. Meiners | T. Debener | G. Schweizer | T. Winkelmann
R. Gharesheikhbayat | L. Dondini | D. Bassi | S. Sansavini
M.J. Rodríguez-Maza | A. Garcés-Claver | M.S. Arnedo-Andrés
X. Xiang | Q.M. Sun | L.X. Ou | J.S. Zhao | J.Z. Chen | C.H. Cai
L. del Piano | M. Abet | C. Sorrentino | M. Sicignano | T. Enotrio
L. del Piano | C. Capone | M. Abet | C. Sorrentino | M. Sicignano | T. Enotrio
N. Mahna
J. Zhou | J.-X. Fu | Z.-X. Wu | S.-S. Huang | Y.-H. Zhao | B. Zhang | Y.-L. Hu | G.-B. Hu | C.-M. Liu
N. Mahna | F. Akbari | S. Sadeghi
C. Fernandez-Caballero | M.I. Escribano | C. Merodio | M.T. Sanchez-Ballesta
Y. Aka Kacar | O. Şimşek | Y. Yalçın Mendi | B. Yıldırım | T. Yeşiloğlu | G. Distefano | S. La Malfa | M. Boncuk
G. Wang-Pruski | S. Babin | P. Murphy | F. Kong | Y. Wu | K. Douglass | A. Murphy | D. De Koeyer | D. Pinto
A.Y. Elsayed | D. da Silva Henriques | E.S.G. Mizubuti | C.P. Carneiro
B. Esfandiyari | G.H. Davarynejad | F.A. Shahriyari | M. Kiani
M. Trandafirescu | I. Trandafirescu | E. Doroftei
K. Beppu | K. Konishi | I. Kataoka
Y. Hayashi | K. Yamada | K. Shiratake | S. Matsumoto
E. Kafkas | S. Paydas Kargi | K.U. Yilmaz | M. Naim Demirtaş
Li Ying Yan | Xiao Fei Song | Xiao Li Li | Zhi Hong Feng | Qun Feng Lou | Jin Feng Chen
C. Uematsu | K. Uozumi | I. Makino | O. Arakawa | H. Katayama | C. Martin
H. Alegría-Mundo | L. Yong | A. Cruz-Ramírez | L. Herrera-Estrella | A. Cruz-Hernández
J.A. Dieleman | J.J. Magan | A.M. Wubs | A. Palloix | S. Lenk | C. Glasbey | F.A. van Eeuwijk
M.-V. Hanke | H. Flachowsky
A. Rival | E. Jaligot | T. Beulé
T.L. Kamps | G.A. Moore
S.V. Siar | R.A. Drew | R.M. Razali | V.N. Villegas
J.-L. Lei | D. Wang | H. Cao | L.-S. Xie | Y.-M. Wu | S. Liu | D.-G. Huang
H. Flachowsky | A. Peil | M.-V. Hanke | C. Tränkner | I. Szankowski | S. Lehmann
T. Sidorova | D. Miroshnichenko | G. Tjukavin | S. Dolgov
L. Yang | L. Li | Y. Xie | Z. Deng | Z. Ye
J.B. Mythili | P.R. Rajeev | G. Ganeshan | P. Chowdappa | S. Yadav | P.H. Ramanjini Gowda
M. Berber | M. Yildiz | K. Abak
K. Breitenbücher | G. Weber | M. Höhnle
L. Girolomini | S. Sabbadini | B. Mezzetti | D. Palma | T. Pandolfini | A. Polverari
M.A. Mazri | I. Belkoura | F. Pliego-Alfaro | M. Belkoura
N. Alburquerque | L. Faize | H. Wang | L. Burgos
M.M. Rego | E.R. Rego | L.P. Farias Filho
J.B. Mythili | P.R. Rajeev | B.S. Chethana
E. Menéndez | V. Marfa | E. Claveria | I. Martínez | M. Pujol | R. Dolcet-Sanjuan
R.C. García-Almodóvar | C. Petri | I.M.G. Padilla | L. Burgos
A. Ilczuk | H. Mibus | M. Schmidt | M. Serek
R. Mikhailov | A. Firsov | O. Shulga | S. Dolgov
Tejaswini | P. Madhavilatha | S. Anuradha | M.V. Dhananjaya | S. Sudhagar
S.C. Debnath