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TRANSFORMATION OF A NOVEL DROUGHT-RESPONSE TRANSCRIPTION FACTOR GENE PEDREB2B INTO WHITE CLOVER VIA SOAKING SEEDS WITH AGROBACTERIUM TUMEFACIENS

Article number
929_49
Pages
343 – 350
Language
English
Abstract
A novel drought-response transcription factor gene PeDREB2b cloned from Populus euphratica Oliv. was successfully transferred into white clover (Trifolium repens L.) ‘Hai Fa’ by soaking seeds with Agrobacterium tumefaciens. In total, 52 kanamycin-resistant primary transformants were obtained.
PCR analysis confirmed that 6 of them carry the PeDREB2b gene.
Southern blotting and GUS assay confirmed that that the PeDREB2b gene had been integrated into the plant genome.
The transformation efficiency was 0.96%, calculated on the basis of the total number of the germinated seeds.
The transgenic plants showed significant stronger drought-tolerance than non-transgenic plants.
Our research suggested that the transformation method of soaking seeds with A. tumefaciens is effective for white clover and PeDREB2b can be used as an approach to enhance drought tolerance in white clover.

Publication
XXVIII International Horticultural Congress on Science and Horticulture for People (IHC2010): International Symposium on Genomics and Genetic Transformation of Horticultural Crops
Authors
J.-L. Lei, D. Wang, H. Cao, L.-S. Xie, Y.-M. Wu, S. Liu, D.-G. Huang
Keywords
white clover, Agrobacterium tumefaciens, genetic transformation, PeDREB2b gene, drought-tolerance
Full text
https://doi.org/10.17660/ActaHortic.2012.929.49
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