OPTIMIZATION OF CHROMOSOMAL PREPARATION AND CYTOLOGICAL ANALYSIS OF IN VITRO CULTURED AFRICAN VIOLET (SAINTPAULIA IONANTHA WENDL.)

African violet has tiny chromosomes that have a tendency to stick together, so the reorganization of centromere is difficult.
This investigation was carried out to introduce staining method.
Leaf explants were cultured in basal MS medium supplemented with NAA (0.2 mg/L) and BAP (1 mg/L) to produce adventitious shoots.
The explants were then subcultured in hormone-free basal MS medium in order to form fine roots and 8-14 mm of root tips were collected.
For the staining of the chromosomes, three methods were applied.
The results showed that clear slides were acquired when roots were pretreated by 8-hydroxyquinoline citrate for 4 hours at room temperature and in the fixing step, they were transferred to fresh carnoy solution for 30 minutes.
They were rinsed with water and finally 2 mm of root tips were stained by acetoorcein to show best metaphase chromosomes.
Karyotype analysis showed 2n=2x=30 chromosomal numbers of the samples and total genome was 29.995 microns.
The ratio of the longest to the smallest chromosome was 2.77.

XXVIII International Horticultural Congress on Science and Horticulture for People (IHC2010): International Symposium on Advances in Ornamentals, Landscape and Urban Horticulture

M. Farjadi-Shakib, A. Mousavi , R. Naderi

acetoorcein, chromosome, cytogenetic, karyotype, ideogram

https://doi.org/10.17660/ActaHortic.2012.937.113