CULTIVAR IDENTIFICATION BY RAPD ANALYSIS IN EPIDENDRUM

Epidendrum belongs to one of the orchid families; it has 700 species and distributes from central and south America.
As for its morphology, there are two characteristics, i.e., long and short stem types.
Cultivar identification of Epidendrum has been tried by morphological characteristics, especially by stem length.
However, since stem length changes depending on environmental factors and the length resembles between genetically close cultivars, it has been difficult to distinguish the cultivars according to morphological characteristics.
In this study, 16 cultivars of Epidendrum were identified by RAPD analysis using seven primers.
DNA was extracted by modified CTAB method from 16 cultivars, and electrophoresis was carried out with HindIII and got template DNA. Then, RAPD was carried out using 7 random primers (OPR-13, -16, OPE-02,-06, -14, -20, OPA-03). All the primers could get positive band sets and 81 amplified fragments were obtained by PCR, resulting that 71 fragments showed polymorphism among the 16 cultivars.
These findings suggest that Epidendrum has genetically wide variation.
The coefficient value ranged from 0.53 to 0.86, and the cultivars were classified into 3 major groups.
In this case, no consistent characteristics in morphology appeared along with each group, however, it is possible to distinguish all the cultivars more than one band difference.
From these findings, it is suggested that the Epidendrum cultivars can be distinguished by RAPD analysis using 7 primer sets in this study.
This RAPD analysis is available for correct identification of the cultivars rather than morphological distinction.

XXVIII International Horticultural Congress on Science and Horticulture for People (IHC2010): International Symposium on Advances in Ornamentals, Landscape and Urban Horticulture

Y. Li, H. Liu, Y. Matsubara

7 primers, polymorphism, coefficient value, dendrogram

https://doi.org/10.17660/ActaHortic.2012.937.73