Articles
ANTIOXIDANT ACTIVITIES OF BULGARIAN GOLDEN ROOT – ENDANGERED MEDICINAL SPECIES
Article number
955_20
Pages
149 – 154
Language
English
Abstract
Rhodiola rosea (Golden Root, Roseroot or Arctic Root) is a yellow-flowered herbaceous cool climate species distributed in the sub-arctic areas of the northern hemisphere (North America, Greenland, Iceland, Scandinavia), in high mountains of Asia (the Altai, the Tien-Shan, and the Himalayas) and in Europe (Central Europe and the Balkan peninsula) above 1800 m.
In Bulgaria, this species is endangered and is included in the Red Book.
It is protected by law in several other countries (e.g.
UK, Finland, Russia, Mongolia). Rhodiola is a multipurpose medicinal herb with adaptogenic properties.
Its radix and rhizome extracts increase the bodys nonspecific resistance and normalize body functions, having antidepressant, anticancer, cardio protective and central nervous system stimulating effects.
The present study aims at establishing the optimal in vitro conditions for in vitro cultivation to stimulate bioactive substances production.
Calli were cultivated on various phytoregulators and concentrations media.
Total phenolic/flavonoid content and radical scavenging activity was determined in these calli.
Antioxidant properties were influenced by the composition of the cultivation media.
A good correlation was obtained between the scavenging activity of 1,1-diphenyl-2-picrylhydrazyl radical with both the total phenolic content and the total flavonoid content.
No strict relation between the callus colour/structure/texture and the secondary metabolite amounts was found.
Despite the low accumulation of phenolic and flavonoid compounds in the calli of Bulgarian R. rosea, the presence of these metabolites indicates the possibility for the production of biologically active substances by non differentiated cells.
This could be a basis for in vitro metabolic engineering and biotransformation for alternative production of valuable substances in Roseroot.
In Bulgaria, this species is endangered and is included in the Red Book.
It is protected by law in several other countries (e.g.
UK, Finland, Russia, Mongolia). Rhodiola is a multipurpose medicinal herb with adaptogenic properties.
Its radix and rhizome extracts increase the bodys nonspecific resistance and normalize body functions, having antidepressant, anticancer, cardio protective and central nervous system stimulating effects.
The present study aims at establishing the optimal in vitro conditions for in vitro cultivation to stimulate bioactive substances production.
Calli were cultivated on various phytoregulators and concentrations media.
Total phenolic/flavonoid content and radical scavenging activity was determined in these calli.
Antioxidant properties were influenced by the composition of the cultivation media.
A good correlation was obtained between the scavenging activity of 1,1-diphenyl-2-picrylhydrazyl radical with both the total phenolic content and the total flavonoid content.
No strict relation between the callus colour/structure/texture and the secondary metabolite amounts was found.
Despite the low accumulation of phenolic and flavonoid compounds in the calli of Bulgarian R. rosea, the presence of these metabolites indicates the possibility for the production of biologically active substances by non differentiated cells.
This could be a basis for in vitro metabolic engineering and biotransformation for alternative production of valuable substances in Roseroot.
Authors
K. Tasheva , G. Kosturkova, A. Trendafilova
Keywords
Rhodiola rosea, Roseroot, phytoregulators, antioxidants
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