Articles
REGENERATION AND MULTIPLICATION OF MAPS GENETIC RESOURCES BY USING TECHNICAL ISOLATION AND CONTROLLED POLLINATION
Article number
955_27
Pages
191 – 196
Language
English
Abstract
Germplasm collection of medicinal, aromatic plants (MAPs) maintained at the Department of Genetic Resources for Vegetables, Medicinal and Special Plants in Olomouc (Czech Republic) comprises about 833 accessions.
Genera Origanum, Betonica, Plantago, Agrimonia, Hypericum, Thymus, Digitalis represent valuable sources for biodiversity conservation and potential materials for researchers and breeders.
The gene bank collection of MAPs includes original seed samples as well as accessions multiplied following the international standards.
The majority of species are allogamous and insect-pollinated.
Multiplication and controlled pollination is needed for the acquisition of good-quality seed set (conservation of species specific genotypes, seed set identity and high germination rate). Two types of pollinators have been tested during two vegetative periods: honey-bees (Apis mellifera) and bumble-bees (Bombus terrestris). Quantity and quality of seed set were assessed in the model species wood betony (Betonica officinalis). The results showed that the highest yield of seed set was recorded in plants pollinated by bumble-bees (24.53 and 26.87 g, resp.), in both years of the experiment, followed by plants pollinated by honey-bees and open-pollinated plants (control). The highest weight of 1000 seeds was observed in plants pollinated by honey-bees (1.11 and 1.15 g, resp.). Mean germination capacity of seeds pollinated by bumble-bees ranged from 15 to 21%, and by honey-bees from 16 to 20%.
Genera Origanum, Betonica, Plantago, Agrimonia, Hypericum, Thymus, Digitalis represent valuable sources for biodiversity conservation and potential materials for researchers and breeders.
The gene bank collection of MAPs includes original seed samples as well as accessions multiplied following the international standards.
The majority of species are allogamous and insect-pollinated.
Multiplication and controlled pollination is needed for the acquisition of good-quality seed set (conservation of species specific genotypes, seed set identity and high germination rate). Two types of pollinators have been tested during two vegetative periods: honey-bees (Apis mellifera) and bumble-bees (Bombus terrestris). Quantity and quality of seed set were assessed in the model species wood betony (Betonica officinalis). The results showed that the highest yield of seed set was recorded in plants pollinated by bumble-bees (24.53 and 26.87 g, resp.), in both years of the experiment, followed by plants pollinated by honey-bees and open-pollinated plants (control). The highest weight of 1000 seeds was observed in plants pollinated by honey-bees (1.11 and 1.15 g, resp.). Mean germination capacity of seeds pollinated by bumble-bees ranged from 15 to 21%, and by honey-bees from 16 to 20%.
Authors
K. Dusek, E. Duskova , I. Dolezalova
Keywords
bumble-bees, honey-bees, insect-pollinated plants, medicinal, aromatic and culinary plants, protection and conservation of germplasm, wood betony
Online Articles (54)