Articles
THE RESTORATION OF ARNICA MONTANA POPULATIONS FROM ARGES COUNTY FLORA USING IN VITRO MICROPROPAGATION
Article number
955_44
Pages
297 – 301
Language
English
Abstract
As part of a project regarding the chorological study of sozological categories for Arges county flora, in vitro propagation technology was developed here for Arnica Montana in order to restore endangered populations through conventional and biotechnological methods of propagation.
The established nutritive media is: for initiation base medium MS, with ½ n, 0.005:2 mg/L auxin/cytokinin, 20 mg/L NaFeEDTA, 20 g/L sucrose, 7g/L agar; for multiplication base medium LF, with n concentration, 0.02:0.4 mg/L auxin/cytokinin, 32 mg/L NaFeEDTA, supplemented with 40 g/L dextrose and 7 g/L agar; for rooting the usage of ½ n macro and micronutrients MS, n vitamins LS, 0.1 mg/L GA3, 0.2 mg/L IBA, 38 mg/L NaFeEDTA, 30 g/L dextrose, 7 g/L agar and 0.3 g/L activated carbon.
The explants obtained in laboratory were acclimatized in a greenhouse and then in a pilot-plot in the natural habitats.
The plants obtained by the micropropagation technique will be in part utilized for medicinal and ornamental purposes, to reduce in this way the abusive gathering of the wild plants and consequently conserving the biodiversity.
The established nutritive media is: for initiation base medium MS, with ½ n, 0.005:2 mg/L auxin/cytokinin, 20 mg/L NaFeEDTA, 20 g/L sucrose, 7g/L agar; for multiplication base medium LF, with n concentration, 0.02:0.4 mg/L auxin/cytokinin, 32 mg/L NaFeEDTA, supplemented with 40 g/L dextrose and 7 g/L agar; for rooting the usage of ½ n macro and micronutrients MS, n vitamins LS, 0.1 mg/L GA3, 0.2 mg/L IBA, 38 mg/L NaFeEDTA, 30 g/L dextrose, 7 g/L agar and 0.3 g/L activated carbon.
The explants obtained in laboratory were acclimatized in a greenhouse and then in a pilot-plot in the natural habitats.
The plants obtained by the micropropagation technique will be in part utilized for medicinal and ornamental purposes, to reduce in this way the abusive gathering of the wild plants and consequently conserving the biodiversity.
Authors
M. Duţă, M. Neblea , A. Teodorescu
Keywords
acclimatization, initiation, multiplication, rooting
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