Articles
INFLUENCE OF 6-BENZYLAMINOPURINE AND INDOLE-3-BUTYRIC ACID ON IN VITRO PROPAGATION AND SECONDARY METABOLITES ACCUMULATION IN LAMIUM ALBUM FROM LOZEN MOUNTAIN
Article number
955_47
Pages
315 – 320
Language
English
Abstract
The effect of different concentrations (0.1-1.0 mg/L) of cytokinin BA (6-benzylaminopurine) and auxin IBA (indole-3-butyric acid) on the in vitro propagation and on the amount of secondary metabolites accumulation as well as their antioxidant capacity in the medicinal plant Lamium album was examined.
A slight increase in shoot numbers was observed on Murashige and Skoogs (MS) medium supplemented with 0.8 mg/L BA when compared to control plants that were propagated on regular MS medium.
All concentrations of BA suppressed root formation and stimulated callogenesis.
Significant root formation on the MS medium supplemented with different concentrations of IBA was observed, especially at 0.7 mg/L IBA. Shoots cultivated on MS medium supplemented with BA from 0.6 up to 0.8 mg/L and IBA in concentration of 0.9 mg/L showed enhanced content of total phenols and flavonoids.
The highest values of antioxidant activity were achieved on MS medium with 0.8 mg/L BA and 0.9 mg/L IBA. Compared to shoots and in vivo growing plants, significant stimulation of total phenolic content and antioxidant activity in forming callus and roots occurred on MS medium supplemented with 0.2, 0.5, 0.8 mg/L BA and 0.2, 0.5, 0.7 mg/L IBA. The amount of flavonoids in developed callus was twofold lower than the one observed in shoots.
As far as we know, this is the first report to indicate stimulation activity of BA and IBA on the production and accumulation of phenolic compounds with high antioxidant capacity, especially in roots and callus, in in vitro propagated Lamium album.
A slight increase in shoot numbers was observed on Murashige and Skoogs (MS) medium supplemented with 0.8 mg/L BA when compared to control plants that were propagated on regular MS medium.
All concentrations of BA suppressed root formation and stimulated callogenesis.
Significant root formation on the MS medium supplemented with different concentrations of IBA was observed, especially at 0.7 mg/L IBA. Shoots cultivated on MS medium supplemented with BA from 0.6 up to 0.8 mg/L and IBA in concentration of 0.9 mg/L showed enhanced content of total phenols and flavonoids.
The highest values of antioxidant activity were achieved on MS medium with 0.8 mg/L BA and 0.9 mg/L IBA. Compared to shoots and in vivo growing plants, significant stimulation of total phenolic content and antioxidant activity in forming callus and roots occurred on MS medium supplemented with 0.2, 0.5, 0.8 mg/L BA and 0.2, 0.5, 0.7 mg/L IBA. The amount of flavonoids in developed callus was twofold lower than the one observed in shoots.
As far as we know, this is the first report to indicate stimulation activity of BA and IBA on the production and accumulation of phenolic compounds with high antioxidant capacity, especially in roots and callus, in in vitro propagated Lamium album.
Authors
M.A. Dimitrova, Z.P. Yordanova , V.M. Kapchina-Toteva
Keywords
micropropagation, phenolic content, flavonoids, antioxidant activity, medicinal plant
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