Articles
GENETICS AND GENOMICS OF THE GLOBE ARTICHOKE: WHERE HAVE WE GOT TO? – RECENT ADVANCES AT CNR-IGV, ITALY
Article number
983_22
Pages
173 – 177
Language
English
Abstract
Globe artichoke, a diploid species (2n=2x=34) belonging to the Asteraceae family, is largely cultivated in the Mediterranean area and in other regions throughout the world, where it is used as a vegetable and for the extraction of bioactive compounds.
Notwithstanding its economic importance, only recently a great boost has been given to the genetics and genomics studies of this crop.
Genetic maps have been constructed on the basis of crosses between globe artichoke cultivars, or between globe artichoke and either its wild progenitor or cultivated cardoon.
Within the globe artichoke × wild cardoon map, several genes, mainly belonging to the caffeoylquinic acid pathway, and functional microsatellite (SSR) markers were mapped.
Most SSRs were retrieved from the globe artichoke EST collection in the NCBI database, and therefore this map represents a first step towards the construction of a functional map in globe artichoke and integrates previously mapped markers, providing a characterization of the 17 linkage groups.
A BAC library was constructed from globe artichoke genomic DNA and is being analysed for markers, using a strategy based on BAC superpool screening.
The availability of a BAC library will allow to link the genetic map of globe artichoke to its physical map.
Moreover, we sequenced the globe artichoke genome at a coverage of 2.3× by using Illumina technology and this allowed to detect and characterize the fraction of repetitive elements in order to infer the organization of the globe artichoke genome, also in comparison to the genome of the globe artichoke wild relative.
These genomic records were also used to derive microRNA precursor and target genes.
Altogether, these data provide a quite updated picture on the advances in the genetic and genomic research and resource availability for the globe artichoke at CNR-IGV, Bari, Italy.
Notwithstanding its economic importance, only recently a great boost has been given to the genetics and genomics studies of this crop.
Genetic maps have been constructed on the basis of crosses between globe artichoke cultivars, or between globe artichoke and either its wild progenitor or cultivated cardoon.
Within the globe artichoke × wild cardoon map, several genes, mainly belonging to the caffeoylquinic acid pathway, and functional microsatellite (SSR) markers were mapped.
Most SSRs were retrieved from the globe artichoke EST collection in the NCBI database, and therefore this map represents a first step towards the construction of a functional map in globe artichoke and integrates previously mapped markers, providing a characterization of the 17 linkage groups.
A BAC library was constructed from globe artichoke genomic DNA and is being analysed for markers, using a strategy based on BAC superpool screening.
The availability of a BAC library will allow to link the genetic map of globe artichoke to its physical map.
Moreover, we sequenced the globe artichoke genome at a coverage of 2.3× by using Illumina technology and this allowed to detect and characterize the fraction of repetitive elements in order to infer the organization of the globe artichoke genome, also in comparison to the genome of the globe artichoke wild relative.
These genomic records were also used to derive microRNA precursor and target genes.
Altogether, these data provide a quite updated picture on the advances in the genetic and genomic research and resource availability for the globe artichoke at CNR-IGV, Bari, Italy.
Authors
G. Sonnante
Keywords
Cynara cardunculus, globe artichoke, wild cardoon, genetic map, genomic sequencing
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