Articles
VIRUS ELIMINATION IN ARTICHOKE: COMPARISON BETWEEN DIFFERENT METHODS OF EXTRACTION AND DETECTION PROCEDURES
Article number
983_41
Pages
295 – 299
Language
English
Abstract
Different extraction and detection methods were compared to evaluate the virus status of artichoke plants produced in vitro.
These diagnostic procedures were performed to assess the efficacy of in vitro thermotherapy and/or meristem tips culture for obtaining virus-free plants.
The viruses studied were: Artichoke Italian latent virus (AILV), Artichoke latent virus (ArLV), and Tomato spotted wilt virus (TSWV). In our experience, the most suitable detection method for in vivo analysis proved to be the tissue print hybridization on nylon membranes.
This procedure is sufficiently sensitive and reliable, but at the same time less expensive than the other methods tested.
When testing materials obtained in vitro, sometimes this method is not sufficiently sensitive, and false negatives frequently appear.
The results of meristem tip culture showed that the excision of apices alone is generally insufficient to obtain AILV-free plants (3.3%), but better results were obtained for ArLV elimination (78.9% virus free plants ). The effectiveness of thermotherapy followed by meristem tip culture reached 100% for AILV and 0% for ArLV. For TSWV we have the result only of meristem tip culture, with percentage recovery of 98.51% (first Trial, subsequently abbreviated T1) and 45.95% (second Trial, subsequently abbreviated, T1R).
These diagnostic procedures were performed to assess the efficacy of in vitro thermotherapy and/or meristem tips culture for obtaining virus-free plants.
The viruses studied were: Artichoke Italian latent virus (AILV), Artichoke latent virus (ArLV), and Tomato spotted wilt virus (TSWV). In our experience, the most suitable detection method for in vivo analysis proved to be the tissue print hybridization on nylon membranes.
This procedure is sufficiently sensitive and reliable, but at the same time less expensive than the other methods tested.
When testing materials obtained in vitro, sometimes this method is not sufficiently sensitive, and false negatives frequently appear.
The results of meristem tip culture showed that the excision of apices alone is generally insufficient to obtain AILV-free plants (3.3%), but better results were obtained for ArLV elimination (78.9% virus free plants ). The effectiveness of thermotherapy followed by meristem tip culture reached 100% for AILV and 0% for ArLV. For TSWV we have the result only of meristem tip culture, with percentage recovery of 98.51% (first Trial, subsequently abbreviated T1) and 45.95% (second Trial, subsequently abbreviated, T1R).
Authors
M. Testa, M. Cadinu, R. Pilia, R. Pintore, L. Baghino
Keywords
meristem tip culture, thermotherapy, ArLV, AILV, TSWV
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