Articles
EFFECT OF DECAPITATION AND SIZE OF EXPLANTS ON IN VITRO MULTIPLICATION RATE OF GLOBE ARTICHOKE
Article number
983_45
Pages
325 – 329
Language
English
Abstract
Growing demand for plants of globe artichoke (Cynara cardunculus var. scolymus L.) made it necessary to find a rapid method of multiplication for this plant.
In vitro micropropagation is an alternative procedure for obtaining healthy, high quality and uniform clones, important to increase the cultivation area of this species.
The contamination problem and low in vitro multiplication rate still remain the limiting factors.
The objective of this study is to obtain healthy plants of globe artichoke via in vitro culture with a high multiplication rate.
In this work, we report an efficient protocol for in vitro multiplication of globe artichoke using decapitated plantlets (removal of apical buds, leaves and roots) and axillary shoots as explants for multiple bud induction.
The size of shoots that may control shoot induction was studied.
To establish the in vitro culture, plants derived from disinfected seeds were cultivated on MS medium containing 1 mg/L IBA and 0.1 mg/L GA3, followed by their decapitation and subculture on proliferation medium containing 1 mg/L kinetin and 0.1 mg/L NAA. In order to study the influence of explant size on shoot proliferation, four shoots size categories (2 cm) were evaluated.
The results show that decapitation of seedlings influenced the efficiency of shoot induction.
The greatest propagation ratio (shoot proliferation) was obtained with decapitated explants (17 shoots per explant). The multiplication rate obtained in this work over twelve generations was 7.56. Statistically significant differences were observed for proliferation among the four shoot size categories and the highest rate obtained with those of 1-1.5 cm in size.
In vitro micropropagation is an alternative procedure for obtaining healthy, high quality and uniform clones, important to increase the cultivation area of this species.
The contamination problem and low in vitro multiplication rate still remain the limiting factors.
The objective of this study is to obtain healthy plants of globe artichoke via in vitro culture with a high multiplication rate.
In this work, we report an efficient protocol for in vitro multiplication of globe artichoke using decapitated plantlets (removal of apical buds, leaves and roots) and axillary shoots as explants for multiple bud induction.
The size of shoots that may control shoot induction was studied.
To establish the in vitro culture, plants derived from disinfected seeds were cultivated on MS medium containing 1 mg/L IBA and 0.1 mg/L GA3, followed by their decapitation and subculture on proliferation medium containing 1 mg/L kinetin and 0.1 mg/L NAA. In order to study the influence of explant size on shoot proliferation, four shoots size categories (2 cm) were evaluated.
The results show that decapitation of seedlings influenced the efficiency of shoot induction.
The greatest propagation ratio (shoot proliferation) was obtained with decapitated explants (17 shoots per explant). The multiplication rate obtained in this work over twelve generations was 7.56. Statistically significant differences were observed for proliferation among the four shoot size categories and the highest rate obtained with those of 1-1.5 cm in size.
Authors
R. El Boullani, A. Elmoslih, A. El Finti, A. El Mousadik, M.A. Serghini
Keywords
Cynara cardunculus var. scolymus L., micropropagation, axillary shoot induction, decapitation, multiplication rate
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