Articles
CALLOGENESIS CAPABILITY OF ARTICHOKE (CYNARA CARDUNCULUS VAR. SCOLYMUS L. FIORI)
Article number
983_54
Pages
377 – 380
Language
English
Abstract
Globe artichoke heads with tightened bracts, in early stage of development, were cultured on medium to produce secondary metabolites through in vitro callus induction.
The culture medium, supplemented with 3.0% sucrose and 0.7% agar, was enriched with naftalenacetic acid (NAA), 6-benzylaminopurine (BAP) and gibberellic acid (GA3) at different concentrations.
Explants were superficially disinfested by immersing them in 70% (v/v) ethanol for 2 minutes and then in 10% of 0.4% (w/v) NaOCl for 20 minutes or in 0.05% (w/v) mercuric chloride for 20 minutes.
Subsequently all the explants were rinsed repeatedly in a solution of 50 mg L-1 ascorbic acid in distilled-sterile water.
The cultures were placed in sterile petri plates and incubated in a growth chamber at 23±1°C in the dark or light and dark (16/8 h) condition.
The substrate was renewed every 30-40 days for the increase in biomass.
Callogenesis varied in response to hormonal concentrations and combinations.
Callus formation was excellent using the combinations with the three growth regulators.
According to this, the presence of auxin is indispensable to promote callogenesis in artichoke, while no significant statistical differences were observed between the explants grown in the dark and under 16 h photoperiod.
The highest callus growth was registered in the dark by applying the lowest concentration of auxin, cytokinin and gibberellic acid.
The results of this study show an efficient protocol for artichoke callus production.
The culture medium, supplemented with 3.0% sucrose and 0.7% agar, was enriched with naftalenacetic acid (NAA), 6-benzylaminopurine (BAP) and gibberellic acid (GA3) at different concentrations.
Explants were superficially disinfested by immersing them in 70% (v/v) ethanol for 2 minutes and then in 10% of 0.4% (w/v) NaOCl for 20 minutes or in 0.05% (w/v) mercuric chloride for 20 minutes.
Subsequently all the explants were rinsed repeatedly in a solution of 50 mg L-1 ascorbic acid in distilled-sterile water.
The cultures were placed in sterile petri plates and incubated in a growth chamber at 23±1°C in the dark or light and dark (16/8 h) condition.
The substrate was renewed every 30-40 days for the increase in biomass.
Callogenesis varied in response to hormonal concentrations and combinations.
Callus formation was excellent using the combinations with the three growth regulators.
According to this, the presence of auxin is indispensable to promote callogenesis in artichoke, while no significant statistical differences were observed between the explants grown in the dark and under 16 h photoperiod.
The highest callus growth was registered in the dark by applying the lowest concentration of auxin, cytokinin and gibberellic acid.
The results of this study show an efficient protocol for artichoke callus production.
Authors
C. Ruta, A. Tagarelli, A. Campanelli, G. De Mastro, I. Morone-Fortunato
Keywords
in vitro culture, secondary metabolite, naftalenacetic acid, 6-benzylaminopurine, gibberellic acid
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