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DETECTION OF TULIP-BREAKING VIRUS IN TULIPS BY THE ENZYME – LINKED IMMONOSORBENT ASSAY (ELISA)

Article number
109_70
Pages
473 – 474
Language
Abstract
As part of the quality improvement and inspection program of the Netherlands Bulb Inspection Service, a test was needed for the serological diagnosis of viruses, including tulip-breaking virus (TBV) in tulips.

The enzyme-linked immunosorbent assay (ELISA) proved to be a sensitive test.

Special precautions are necessary for the preparation of a TBV anti-serum, such as serological purification of the TBV suspension by the complete removal of contaminations, i.e., lily symptomless virus (LSV) and fraction-1-proteins, with anti-LSV and anti-F1 sera.
During the growing season, TBV was detected in tulip leaves and bulbs by ELISA. During storage, TBV could be detected in bulbs from primarily infected tulips.

Publication
III International Symposium on Flower Bulbs
Authors
A.R. van Schadewijk, J. Eggink
Keywords
Full text
https://doi.org/10.17660/ActaHortic.1980.109.70
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