Articles
Medium composition, coconut water, and chitosan effects on Phalaenopsis protocorm growth and development
Article number
1262_18
Pages
133 – 140
Language
English
Abstract
Protocorm multiplication through the regeneration of protocorm-like bodies (PLBs) is used to obtain clonal progenies of hybrid orchids.
The aim of the present study was to identify effective media for commercial orchid propagation through PLB proliferation.
We evaluated the effects of basal media and either coconut water or chitosan supplementation on the growth of PLBs and the proliferation of Phalaenopsis hybrid PLBs, using two experiments, with random complete block designs (RBCD). The first experiment evaluated the progeny of white (V3) × pink standard hybrid (KHM0421, population 1) and their reciprocal hybridization (population 2) and different medium compositions.
The hybrid seeds germinated and developed into protocorms on a half-strength Murashige and Skoog (½ MS) medium supplemented with 15% coconut water (CW). The protocorms were then transferred to four different media: 1) ½ MS; 2) ½ MS + 15% CW; 3) 2 g L‑1 complete fertilizer (CF); and 4) CF + 15% CW. Experiment two evaluated the effects of chitosan on the growth and proliferation of protocorms from population 1, using four different media: 1) ½ MS; 2) 2 g L‑1 CF; 3) ½ MS + 5 ppm chitosan; and 4) CF + 5 ppm chitosan, supplemented with 15% CW. The first experiment showed that the protocorm from population 1 generated more PLBs (5.4 PLBs in 16 weeks) than that of population 2 (2.2 PLBs in 16 weeks). The best media for protocorm proliferation and plantlet regeneration was CF and CF + 15% CW. The results of the second experiment revealed CF + 15% CW was the best medium for protocorm multiplication and plantlet regenerations.
Chitosan tended to have a negative effect on protocorm growth and did not increase protocorm multiplication or plantlet regeneration.
The use of CF medium could benefit orchid propagation, as it was cheaper than the MS medium.
The aim of the present study was to identify effective media for commercial orchid propagation through PLB proliferation.
We evaluated the effects of basal media and either coconut water or chitosan supplementation on the growth of PLBs and the proliferation of Phalaenopsis hybrid PLBs, using two experiments, with random complete block designs (RBCD). The first experiment evaluated the progeny of white (V3) × pink standard hybrid (KHM0421, population 1) and their reciprocal hybridization (population 2) and different medium compositions.
The hybrid seeds germinated and developed into protocorms on a half-strength Murashige and Skoog (½ MS) medium supplemented with 15% coconut water (CW). The protocorms were then transferred to four different media: 1) ½ MS; 2) ½ MS + 15% CW; 3) 2 g L‑1 complete fertilizer (CF); and 4) CF + 15% CW. Experiment two evaluated the effects of chitosan on the growth and proliferation of protocorms from population 1, using four different media: 1) ½ MS; 2) 2 g L‑1 CF; 3) ½ MS + 5 ppm chitosan; and 4) CF + 5 ppm chitosan, supplemented with 15% CW. The first experiment showed that the protocorm from population 1 generated more PLBs (5.4 PLBs in 16 weeks) than that of population 2 (2.2 PLBs in 16 weeks). The best media for protocorm proliferation and plantlet regeneration was CF and CF + 15% CW. The results of the second experiment revealed CF + 15% CW was the best medium for protocorm multiplication and plantlet regenerations.
Chitosan tended to have a negative effect on protocorm growth and did not increase protocorm multiplication or plantlet regeneration.
The use of CF medium could benefit orchid propagation, as it was cheaper than the MS medium.
Publication
Authors
D. Sukma, S.A. Aziz, N. Andini, H.A. Putri, S. Sudarsono
Keywords
chitosan, coconut water, complete fertilizer (NPK 20:20:20), MS medium, protocorm-like bodies (PLBs)
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