Articles
Enhanced growth of in vitro plantlets by cytokinin and pre-colchicine application in Phalaenopsis amabilis Blume protocorms
Article number
1262_27
Pages
205 – 212
Language
English
Abstract
Polyploidy induction in Phalaenopsis amabilis is one of the important methods to develop broader diversity and expand the genotypes germplasm to include better characters.
Producing a high percentage of polyploidy plants in P. amabilis culture raised a question of how to produce better culture conditions when using protocorms as propagules.
This study aimed to study P. amabilis protocorm growth on different cytokinin compositions before and after colchicine application and its effect on polyploidy.
The study consisted of two experiments laid out in a completely randomized design investigating medium composition, with five levels (half strength Murashige-Skoog medium (½ MS) + 15% of coconut water (CW); ½ MS + 0.51 mg L‑1 BAP; ½ MS + 0.5 mg L‑1 TDZ; ½ MS + 15% of CW + 0.51 mg L‑1 BAP; ½ MS + 15% of CW + 0.5 mg L‑1 TDZ), with three replications.
The result showed that cytokinin applied before colchicine application produced higher multiplication and putative polyploids number on ½ MS + 15% CW + 0.51 mg L‑1 BAP or ½ MS + 0.5 mg L‑1 TDZ medium.
Screening of plantlets based on morphological characters allowed identification of normal and the putative polyploid plantlets.
Chromosome number analysis of putative polyploid plantlets revealed that 20% of plantlets were polyploids.
Producing a high percentage of polyploidy plants in P. amabilis culture raised a question of how to produce better culture conditions when using protocorms as propagules.
This study aimed to study P. amabilis protocorm growth on different cytokinin compositions before and after colchicine application and its effect on polyploidy.
The study consisted of two experiments laid out in a completely randomized design investigating medium composition, with five levels (half strength Murashige-Skoog medium (½ MS) + 15% of coconut water (CW); ½ MS + 0.51 mg L‑1 BAP; ½ MS + 0.5 mg L‑1 TDZ; ½ MS + 15% of CW + 0.51 mg L‑1 BAP; ½ MS + 15% of CW + 0.5 mg L‑1 TDZ), with three replications.
The result showed that cytokinin applied before colchicine application produced higher multiplication and putative polyploids number on ½ MS + 15% CW + 0.51 mg L‑1 BAP or ½ MS + 0.5 mg L‑1 TDZ medium.
Screening of plantlets based on morphological characters allowed identification of normal and the putative polyploid plantlets.
Chromosome number analysis of putative polyploid plantlets revealed that 20% of plantlets were polyploids.
Publication
Authors
S.A. Aziz, A.A. Putri, D. Sukma, M. Syukur
Keywords
colchicine, cytokinin application, medium composition, polyploid, protocorm growth
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