Articles
Callus induction, plant regeneration, and cryopreservation of Dendrobium findlayanum based on an encapsulation-dehydration technique
Article number
1262_20
Pages
149 – 160
Language
English
Abstract
Dendrobium findlayanum, primarily distributed in Thailand and neighbouring countries like Myanmar and Laos, is at risk of extinction due to habitat decline and overexploitation.
Cryopreservation has emerged as a promising method for orchid conservation.
This study was carried out to optimize the conditions for callus induction, plant regeneration, and the cryopreservation of protocorms of D. findlayanum using an encapsulation-dehydration method.
The orchid seeds successfully germinated on Vacin and Went medium.
This medium, amended with 1 mg L‑1 6-benzylaminopurine (6-BA), was the most effective for callus induction, whereas a combination of 1 mg L‑1 6-BA and 1 mg L‑1 naphthaleneacetic acid was sufficient for promoting plantlet regeneration.
Protocorms of D. findlayanum were successfully cryopreserved using the following protocol: 24-h pretreatment of alginate-encapsulated protocorms with 0.5 M sucrose followed by 8 h dehydration.
Random amplification of polymorphic DNA was carried out to detect the genetic stability of the cryopreserved protocorms and genetic differences were not detected between non-cryopreserved and cryopreserved protocorms.
Cryopreservation has emerged as a promising method for orchid conservation.
This study was carried out to optimize the conditions for callus induction, plant regeneration, and the cryopreservation of protocorms of D. findlayanum using an encapsulation-dehydration method.
The orchid seeds successfully germinated on Vacin and Went medium.
This medium, amended with 1 mg L‑1 6-benzylaminopurine (6-BA), was the most effective for callus induction, whereas a combination of 1 mg L‑1 6-BA and 1 mg L‑1 naphthaleneacetic acid was sufficient for promoting plantlet regeneration.
Protocorms of D. findlayanum were successfully cryopreserved using the following protocol: 24-h pretreatment of alginate-encapsulated protocorms with 0.5 M sucrose followed by 8 h dehydration.
Random amplification of polymorphic DNA was carried out to detect the genetic stability of the cryopreserved protocorms and genetic differences were not detected between non-cryopreserved and cryopreserved protocorms.
Publication
Authors
S. Bunnag, W. Pimda
Keywords
callus induction, plant regeneration, orchid, RAPD
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