Articles
The effect of light quality on protocorm-like body induction, cell division, and endopolyploidy occurrence in Phalaenopsis ‘Spring Dancer’
Article number
1262_25
Pages
187 – 194
Language
English
Abstract
In this study, the effect of light quality on embryogenic cell division and growth by control of endoreduplication in Phalaenopsis ‘Spring Dancer’ were studied via protocorm-like bodies (PLB). Various wavelengths of light were used for PLB induction to investigate their effect on embryogenic cell division and endoreduplication occurrence in Phalaenopsis. At the early stage of PLB induction, the percentage of S phase (undergoing DNA synthesis) cells increased and reached the maximum at 14 d of culture under all light treatments.
In particular, the explants exposed to full red light (R60) and 2 red:1 blue:1 green light (R30B15G15) had a higher percentage of cells in the S- and G2M phases, as compared to other light qualities.
The levels of oxidized phenol, which is a stress-induced exudate into the medium from explants, were also lower when exposed to R60 and R30B15G15 in comparison to fluorescent light (FL) and 1 red:1 blue light (R30B30). The decreased rate of blue light (480 nm) may reduce stress and increased red light (680 nm) may stimulate embryogenic cell division.
In particular, the explants exposed to full red light (R60) and 2 red:1 blue:1 green light (R30B15G15) had a higher percentage of cells in the S- and G2M phases, as compared to other light qualities.
The levels of oxidized phenol, which is a stress-induced exudate into the medium from explants, were also lower when exposed to R60 and R30B15G15 in comparison to fluorescent light (FL) and 1 red:1 blue light (R30B30). The decreased rate of blue light (480 nm) may reduce stress and increased red light (680 nm) may stimulate embryogenic cell division.
Publication
Authors
A.R. Kwon, S.Y. Park
Keywords
light quality, endoreduplication, PLB, Phalaenopsis
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