Articles
Different carbon sources on growth and development of Paphiopedilum ‘Delrosi’ shoots
Article number
1262_21
Pages
161 – 166
Language
English
Abstract
Lady’s slipper orchids are a challenge for either in vitro or ex vivo multi-propagation.
This experiment aimed to culture Paphiopedilum ‘Delrosi’ using in vitro propagation.
A single shoot (with 3 leaves) of P. ‘Delrosi’ was individually cultured for 16 weeks on modified Hyponex medium with 4.52 µM 2,4-dichlorophenoxyacetic acid (2,4-D) and supplemented with 0.45 µM thidiazuron (TDZ) or 4.65 µM kinetin and containing 20 g L‑1 of a different carbon source (sucrose, glucose, or maltose). It was found that none of the combinations of media provided significantly affected the percentage (50-80%) of shoot formation from shoot explants; however, 80% shoot formation occurred on medium containing sucrose or glucose, (with 0.45 µM TDZ) providing an average of 1.5 new shoots, (120.5 mg FW) and 1.8 new shoots (162.6 mg FW), respectively.
Adding maltose gave 50-60% shoot formation with 0.5-1.1 new shoots and resulted in less fresh weight (23.8-28.8 mg FW) than that of sucrose.
Our experiment concluded that sucrose and glucose increased fresh weight (1,812.0-1,995.2 mg) and root number (5.4-5.9) more than maltose (914.6-1,137.1 mg FW and 2.5-3.6 roots).
This experiment aimed to culture Paphiopedilum ‘Delrosi’ using in vitro propagation.
A single shoot (with 3 leaves) of P. ‘Delrosi’ was individually cultured for 16 weeks on modified Hyponex medium with 4.52 µM 2,4-dichlorophenoxyacetic acid (2,4-D) and supplemented with 0.45 µM thidiazuron (TDZ) or 4.65 µM kinetin and containing 20 g L‑1 of a different carbon source (sucrose, glucose, or maltose). It was found that none of the combinations of media provided significantly affected the percentage (50-80%) of shoot formation from shoot explants; however, 80% shoot formation occurred on medium containing sucrose or glucose, (with 0.45 µM TDZ) providing an average of 1.5 new shoots, (120.5 mg FW) and 1.8 new shoots (162.6 mg FW), respectively.
Adding maltose gave 50-60% shoot formation with 0.5-1.1 new shoots and resulted in less fresh weight (23.8-28.8 mg FW) than that of sucrose.
Our experiment concluded that sucrose and glucose increased fresh weight (1,812.0-1,995.2 mg) and root number (5.4-5.9) more than maltose (914.6-1,137.1 mg FW and 2.5-3.6 roots).
Publication
Authors
K. Obsuwan, E. Nisayan, A. Thongpakdee, C. Thepsithar
Keywords
lady’s slipper orchids, shoot induction, sucrose, glucose, maltose
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