Articles
The role of CRISPR/Cas9 in postharvest, biotic, and abiotic stress
Article number
1396_10
Pages
67 – 72
Language
English
Abstract
Food security has been currently threatened by rapid increasing population and the limitations of agricultural production due to global climactic change.
Postharvest losses are also an essential contributor of food insecurity.
Short shelf-life of fresh agricultural production leads to enormous amounts of postharvest waste.
Obviously, approximately 35% of fresh product has been lost before consumption.
Improving crop characteristics including disease-resistance, drought-tolerance and resilience to heat and salinity stress as well as extending shelf life could be a serious alternative to face climatic change.
In the latest decades, rapid progress of molecular biology accelerates using genome editing techniques extensively.
Clustered regularly interspaced short palindromic repeats (CRISPR), linked to the CRISPR Associated protein (Cas9) is considered a game changer in genome editing technology, and provides various opportunities to engineer desirable crop traits.
The CRISPR/Cas9 system enables to edit genes in a targeted genome region with high accuracy and efficiency.
With precise genome engineering and transgene-free applications, it is predicted that CRISPR can face main difficulties of crop improvement.
This review will focus on the role of CRISPR in reducing plant stress to biotic and abiotic stress.
Additionally, effect of CRISPER on extending shelf life of some agricultural product will be highlighted in the review.
Postharvest losses are also an essential contributor of food insecurity.
Short shelf-life of fresh agricultural production leads to enormous amounts of postharvest waste.
Obviously, approximately 35% of fresh product has been lost before consumption.
Improving crop characteristics including disease-resistance, drought-tolerance and resilience to heat and salinity stress as well as extending shelf life could be a serious alternative to face climatic change.
In the latest decades, rapid progress of molecular biology accelerates using genome editing techniques extensively.
Clustered regularly interspaced short palindromic repeats (CRISPR), linked to the CRISPR Associated protein (Cas9) is considered a game changer in genome editing technology, and provides various opportunities to engineer desirable crop traits.
The CRISPR/Cas9 system enables to edit genes in a targeted genome region with high accuracy and efficiency.
With precise genome engineering and transgene-free applications, it is predicted that CRISPR can face main difficulties of crop improvement.
This review will focus on the role of CRISPR in reducing plant stress to biotic and abiotic stress.
Additionally, effect of CRISPER on extending shelf life of some agricultural product will be highlighted in the review.
Authors
A.A. Ahmed, G.S. Tofiq, N.J. Qadir, S.A. Qadir
Keywords
molecular scissors, gene editing, cas9, postharvest waste (PHL), biotic stress, food security, shelf life
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