Articles
Investigation of antifungal mechanism of hinokitiol against Botrytis cinerea using gene expression analysis
Article number
1396_13
Pages
91 – 98
Language
English
Abstract
Fungal infestation is one of the major causes of pre and postharvest deterioration of fruits and vegetables which results in great economic losses. Botrytis cinerea, a commercially significant phytopathogen, causes gray mold on over 200 plant species around the world.
In a previous study, it was discovered that hinokitiol strongly inhibited the mycelial growth of B. cinerea in a dose-dependent manner.
However, the inhibitory mechanism remains unknown.
Most of the fungicides act on disrupting the biosynthesis pathway of ergosterol.
Ergosterol maintains the integrity and fluidity of the plasma membrane in fungi.
The present study aims to investigate the effect of hinokitiol on ergosterol content and the control mechanism of ergosterol biosynthesis, particularly to study the mechanism of antifungal activity of hinokitiol against B. cinerea on gene expression involving the ergosterol biosynthesis pathway. B. cinerea spores were treated with different hinokitiol concentrations (0, 10, 30, and 60 mg L‑1) and incubated for 0, 12, 24, and 48 h.
The ergosterol level in B. cinerea (30 and 60 mg L‑1) remained stable at different time intervals, while it increased in the control treatment (0 mg L‑1). The expression levels of 6 genes (BcERG1, BcERG2, BcERG3, BcERG11, PdERG5, and PdERG6) involved in the ergosterol pathway were examined using qRT-PCR. The present research focuses on the mechanism of hinokitiol against B. cinerea through ergosterol biosynthesis.
In a previous study, it was discovered that hinokitiol strongly inhibited the mycelial growth of B. cinerea in a dose-dependent manner.
However, the inhibitory mechanism remains unknown.
Most of the fungicides act on disrupting the biosynthesis pathway of ergosterol.
Ergosterol maintains the integrity and fluidity of the plasma membrane in fungi.
The present study aims to investigate the effect of hinokitiol on ergosterol content and the control mechanism of ergosterol biosynthesis, particularly to study the mechanism of antifungal activity of hinokitiol against B. cinerea on gene expression involving the ergosterol biosynthesis pathway. B. cinerea spores were treated with different hinokitiol concentrations (0, 10, 30, and 60 mg L‑1) and incubated for 0, 12, 24, and 48 h.
The ergosterol level in B. cinerea (30 and 60 mg L‑1) remained stable at different time intervals, while it increased in the control treatment (0 mg L‑1). The expression levels of 6 genes (BcERG1, BcERG2, BcERG3, BcERG11, PdERG5, and PdERG6) involved in the ergosterol pathway were examined using qRT-PCR. The present research focuses on the mechanism of hinokitiol against B. cinerea through ergosterol biosynthesis.
Authors
N. Benyakart, H. Umehara, K. Hridhay Keerthana, T. Vanitha, T. Shiina
Keywords
Botrytis cinerea, ergosterol biosynthesis, gene expression, hinokitiol
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