Articles
Transitional changes of the spatially distributed ROS metabolism in apple fruit during CA storage
Article number
1396_5
Pages
37 – 40
Language
English
Abstract
Low oxygen stress can, depending its severity, induce various levels of ROS production.
Even under normoxic conditions, the diffusion resistance of the fruit skin and underlying tissues induces a gas concentration gradient towards the centre of the fruit.
As a result, hypoxic stress will increase when moving from the outer into inner tissues.
The aim is to study the impact of such spatial gradients especially during the transitional phase of fruit moving from one gas condition to the next.
The spatial distribution of ROS metabolism was evaluated analysing four radial samples of Greenstar apple with flesh samples taken along an equatorial radius.
The main players involved in the ROS metabolism under CA storage were measured, which included O2-, AsA, TPC, SOD, CAT, and APX. Depending on the component different spatial gradients were observed either or not changing over the 7 days of the experiment.
The differences in tissues characteristics and gas gradient between inner and outer samples can explain the typical radial pattern of ROS metabolism in apples under low oxygen stress.
Samples from the different locations were differently prone to oxidative burst indicating different levels of oxidative stress.
This study is a step towards a better understanding on spatial gradients affecting the localized metabolic process in general and it provides detailed insights in the ROS metabolism during low oxygen storage.
Even under normoxic conditions, the diffusion resistance of the fruit skin and underlying tissues induces a gas concentration gradient towards the centre of the fruit.
As a result, hypoxic stress will increase when moving from the outer into inner tissues.
The aim is to study the impact of such spatial gradients especially during the transitional phase of fruit moving from one gas condition to the next.
The spatial distribution of ROS metabolism was evaluated analysing four radial samples of Greenstar apple with flesh samples taken along an equatorial radius.
The main players involved in the ROS metabolism under CA storage were measured, which included O2-, AsA, TPC, SOD, CAT, and APX. Depending on the component different spatial gradients were observed either or not changing over the 7 days of the experiment.
The differences in tissues characteristics and gas gradient between inner and outer samples can explain the typical radial pattern of ROS metabolism in apples under low oxygen stress.
Samples from the different locations were differently prone to oxidative burst indicating different levels of oxidative stress.
This study is a step towards a better understanding on spatial gradients affecting the localized metabolic process in general and it provides detailed insights in the ROS metabolism during low oxygen storage.
Authors
Qianyun Han, M. Hertog, B. Nicolaï
Keywords
apple, low oxygen stress, ROS metabolism, spatial study
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