Articles
Detection of false codling moth (Thaumatotibia leucotreta Meyrick) infestation in ‘Midknight Valencia’ fruit (Citrus sinensis L. Osbeck) using SIFT-MS
Article number
1396_16
Pages
115 – 118
Language
English
Abstract
False codling moth (FCM), Thaumatotibia leucotreta (Meyrick), is a phytosanitary pest capable of attacking various agricultural products including citrus fruit.
As a quarantine insect pest, its presence limits the trade and market of host crops internationally.
Producers have control measures in place in the field, but the absence of infestation is not always guaranteed.
Moreover, detection of FCM infested citrus fruit in packhouses remain challenging especially during the early stages of infestation.
An alternative screening method for FCM infestation is now under investigation.
This study evaluates the applicability of selected ion flow tube mass spectrometry (SIFT-MS) for a rapid and non-destructive detection of FCM infestation in citrus.
In addition, headspace sorptive extraction-gas chromatography-mass spectrometry (HSSE-GC-MS) was used to identify volatile compounds relevant to the infestation. Midknight Valencia orange (Citrus sinensis L. Osbeck) were inoculated with FCM eggs, and infestation was allowed to proceed for 15 days.
Analyses were done in intervals from the early to later stages of larval development.
Using multivariate statistics, the results indicate that discrimination between healthy and infested fruits is possible using SIFT-MS in full scan mode with the discrimination improving at the later stages of infestation.
Furthermore, compounds unique to FCM infested fruit and larvae were identified with HSSE-GC-MS. In conclusion, SIFT-MS potentially can be used for the rapid and non-destructive screening of FCM infested citrus fruits postharvest.
In addition, due to the presence of compounds unique to infested citrus, it may be beneficial to use another SIFT-MS mode, selected ion mode, in which the instrument scans only the ions relevant to those compounds.
With this, discrimination at the early stages may be improved.
As a quarantine insect pest, its presence limits the trade and market of host crops internationally.
Producers have control measures in place in the field, but the absence of infestation is not always guaranteed.
Moreover, detection of FCM infested citrus fruit in packhouses remain challenging especially during the early stages of infestation.
An alternative screening method for FCM infestation is now under investigation.
This study evaluates the applicability of selected ion flow tube mass spectrometry (SIFT-MS) for a rapid and non-destructive detection of FCM infestation in citrus.
In addition, headspace sorptive extraction-gas chromatography-mass spectrometry (HSSE-GC-MS) was used to identify volatile compounds relevant to the infestation. Midknight Valencia orange (Citrus sinensis L. Osbeck) were inoculated with FCM eggs, and infestation was allowed to proceed for 15 days.
Analyses were done in intervals from the early to later stages of larval development.
Using multivariate statistics, the results indicate that discrimination between healthy and infested fruits is possible using SIFT-MS in full scan mode with the discrimination improving at the later stages of infestation.
Furthermore, compounds unique to FCM infested fruit and larvae were identified with HSSE-GC-MS. In conclusion, SIFT-MS potentially can be used for the rapid and non-destructive screening of FCM infested citrus fruits postharvest.
In addition, due to the presence of compounds unique to infested citrus, it may be beneficial to use another SIFT-MS mode, selected ion mode, in which the instrument scans only the ions relevant to those compounds.
With this, discrimination at the early stages may be improved.
Authors
M.C. Cardinez, M. Hertog, S. Moore, B. Nicolaï
Keywords
false codling moth, infestation, volatile, orange
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