Articles
Transcriptome analysis of the effect of packaging conditions on the quality of edamame
Article number
1396_48
Pages
359 – 366
Language
English
Abstract
Edamame, immature, green soybeans, is consumed in many countries in recent years because of its nutritional benefits.
Because of its high respiration rate, the edamame loses its quality very rapidly after harvest.
In the present study, the effect of packaging conditions including modified atmosphere packaging (MAP) on the transcription behavior of edamame was investigated.
Edamame pods with four different packaging conditions (oriented polypropylene (OPP) pouch with eight macro holes of 6 mm in diameter and heat-sealed as the control, CTL; polyethylene net, NET; micro-perforated MA film pouch, MAP; heat-sealed OPP, HS-OPP) were stored at 10°C until 7 days.
A total of 20 samples of total mRNA extracted from edamame seeds stored for 0, 2, 4, and 7 days was analyzed by using microarray for soybean (Glycine max). Data on relative gene expression was analyzed using a microarray data analysis software.
Four genes showing distinct expression patterns depending on the packaging conditions and periods of storage were identified.
Other genes showing similar expression patterns were also selected based on the similarity indexes.
A hierarchical cluster analysis on 100 genes including 4 representative genes succeeded to identify the clusters related to quality alterations depending on the conditions and periods of the storage.
We have previously reported the relationship between the expression of stachyose synthase-encoding gene and the content of stachyose which is one of the senescence marker molecules of edamame.
Further gene ontology (GO) analysis will provide information on other marker genes for quality alteration of edamame.
Because of its high respiration rate, the edamame loses its quality very rapidly after harvest.
In the present study, the effect of packaging conditions including modified atmosphere packaging (MAP) on the transcription behavior of edamame was investigated.
Edamame pods with four different packaging conditions (oriented polypropylene (OPP) pouch with eight macro holes of 6 mm in diameter and heat-sealed as the control, CTL; polyethylene net, NET; micro-perforated MA film pouch, MAP; heat-sealed OPP, HS-OPP) were stored at 10°C until 7 days.
A total of 20 samples of total mRNA extracted from edamame seeds stored for 0, 2, 4, and 7 days was analyzed by using microarray for soybean (Glycine max). Data on relative gene expression was analyzed using a microarray data analysis software.
Four genes showing distinct expression patterns depending on the packaging conditions and periods of storage were identified.
Other genes showing similar expression patterns were also selected based on the similarity indexes.
A hierarchical cluster analysis on 100 genes including 4 representative genes succeeded to identify the clusters related to quality alterations depending on the conditions and periods of the storage.
We have previously reported the relationship between the expression of stachyose synthase-encoding gene and the content of stachyose which is one of the senescence marker molecules of edamame.
Further gene ontology (GO) analysis will provide information on other marker genes for quality alteration of edamame.
Authors
T. Shiina, R. Iida, T. Kitazawa, D. Ciptaningtyas, N. Benyakart, H. Umehara, M. Thammawong, H.A. Naznin, K. Nakano, T. Orikasa, M. Nagata
Keywords
microarray, modified atmosphere packaging, quality, transcriptomics, young soybean
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