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Articles

STUDIES ON NUCLEAR PROTEINS OF TULIP: NUCLEAR ISOLATION AND PROTEIN FRACTIONATION

Article number

23_22

Pages

142 – 149

Language

Abstract

Tulip leaves of cv. ‘Golden Age’ were disrupted by blending in dry ice.
Nuclei were isolated after dehydration in cold acetone by centrifugation in carbon tetrachloride-cyclohexane mixtures of varying densities.
The purest nuclear fraction was obtained at a density between 1. 450 and 1. 470 at 0°C. DNA assays indicate about a five fold purification of nuclei Soluble proteins, histones, chromosome residual proteins, and nuclear residual proteins were prepared and analyzed for protein and DNA content.

Publication

I International Symposium on Flowerbulbs

Authors

W.F. Sheridan

Keywords

Full text

https://doi.org/10.17660/ActaHortic.1971.23.22

Online Articles (72)

2. SOME FACTS ABOUT THE POSITION OF THE DUTCH BULB INDUSTRY

W. van Soest

3. BULBOUS PLANTS IN SCIENTIFIC RESEARCH; PAST, PRESENT AND FUTURE

P.K. Schenk

4. BULBOUS PLANTS WILD IN SOUTH-WEST ASIA

P. Furse

5. BULB GROWING IN THE NETHERLANDS

A.F.G. Slootweg

6. TRANSLOCATION AND DISTRIBUTION OF ASSIMILATES IN FLOWERING FRITILLARIA IMPERIALIS

J. van Die | T. Tietema

7. THE INTERACTION OF TEMPERATURE AND PHOTOPERIOD ON GROWTH AND FLOWERING OF LILIUM LONGIFLORUM THUNB. CV.’NELLIE WHITE’

H.F. Wilkins | E. Waters

8. VEGETATIVE AND FLOWERING RESPONSES OF LILIUM LONGIFLORUM THUNB. CULTIVARS TO COLD AND LONG DAY TREATMENT AS RELATED TO BULB MATURITY

A.N. Roberts | F.W. Moeller

9. THE EFFECTS OF WARM STORAGE ON THE GROWTH AND FLOWERING OF LILIUM LONGIFLORUM (THUNB.) ‘ACE’

R.W. Langhans | T.C. Weiler

10. INFLUENCE OF LIGHT ON FLOWER BUD ABSCISSION IN PLANTS OF THE LILY CULTIVAR ‘ENCHANTMENT’

G.A. Kamerbeek | A.J.B. Durieux

11. CHANGES OF ENDOGENOUS GROWTH SUBSTANCES IN EASTER LILY AS EFFECTED BY COOLING

Y. Tsukamoto

12. ENDOGENOUS GIBBERELLIN LEVEL IN ORNITHOGALUM ARABICUM AND ITS RELATIONSHIP TO STORAGE TEMPERATURES OF BULBS AND TO FLOWER DEVELOPMENT

A.H. Halevy | J. Mor | J. Valershtein

13. ASPECTS OF EXTRACTABLE GIBBERELLIN-LIKE SUBSTANCES FROM TULIPA GESNERIANA L.

A.A. De Hertogh | L. Aung | A.E. Einert | G.L. Staby

14. THE INFLUENCE OF DAYLENGTH ON THE DATE OF FLOWERING, HEIGHT AND CORMSIZE OF GLADIOLUS

E. Alvares Vega

15. EFFECTS OF STORAGE AND DATE OF PLANTING ON FLOWERING AND BULB GROWTH OF NERINE BOWDENII

W. Sytsema

16. TEMPERATURE AND MOISTURE INFLUENCE ON FLOWERING OF EUCHARIS GRANDIFLORA

R.S. Lindstrom

17. INFLUENCE OF HEAT-TREATMENT TEMPERATURES DURING STORAGE ON FLOWER FORMATION AND DEVELOPMENT OF 5°-TULIPS

A.F.G. Slootweg | P. Hoogeterp

18. FLOWER QUALITY IN RELATION TO STORAGE TREATMENTS FOR EARLY FORCING OF NARCISSUS AND TULIP

E.D. Turquand

19. POST-HARVEST DEVELOPMENT OF CUT TULIP FLOWERS¹⁾

M. Benschop | A.A. DeHertogh

20. INFLUENCE OF 8-HYDROXYQUINOLINE CITRATE AND SUCROSE ON CARBOHYDRATE CONTENT OF LEAVES AND FLORETS OF CUT GLADIOLUS SPIKES

F.J. Marousky

21. THE MORPHOLOGY AND PHYSIOLOGY OF BULBOUS PLANTS PAST, PRESENT AND FUTURE

A.R. Rees

22. SOME ASPECTS OF THE RELATIONSHIP BETWEEN THE BIOLOGICAL AND THE ECONOMIC YIELD OF TULIP BULBS

M.J.G. Timmer

23. STUDIES ON NUCLEAR PROTEINS OF TULIP: NUCLEAR ISOLATION AND PROTEIN FRACTIONATION

W.F. Sheridan

24. THE BIOSYNTHESIS OF TERPENES IN CELL-FREE EXTRACTS FROM BULBS AS RELATED TO GROWTH AND DEVELOPMENT*

G.L. Staby | A.A. De Hertogh

25. THE ALTERATION OF BULB HORMONES CONTENT BY ENVIRONMENTAL STIMULI

L.H. Aung

26. SOME METABOLIC FACTORS ASSOCIATED WITH DORMANCY IN TULIPS

D.M. Shaw | E.W. Toop

27. GUMMOSIS OF TULIP BULBS CAUSED BY ETHYLENE

G.A. Kamerbeek | A.L. Verlind | J.A. Schipper

28. METABOLISM OF THE CARBOHYDRATE-PROTEIN CONTENT IN GLADIOLUS

A. Gromov

29. CONTROL OF FUSARIUM AND PENICILLIUM ROTS OF IRIS, TULIP AND NARCISSUS WITH THIABENDAZOLE AND BENOMYL 1)

Ch.J. Gould | V.L. Miller

30. CONTROL OF FUSARIUM IN SOME BULBOUS AND TUBEROUS PLANTS GROWN FOR CUTFLOWER PRODUCTION UNDER GLASS

G. Scholten

31. NEW DEVELOPMENTS IN CONTROLLING BOTRYTIS TULIPAE IN TULIPS

A. W. Doornik | M. de Rooy

32. CONTROL METHODS FOR POST-HARVEST DISEASES OF FLOWERS AND BULBS

R.O. Magie

33. THE INTERACTION OF TEMPERATURE AND FUNGICIDES DURING THE STORAGE OF TULIP BULBS

E. Valásková

34. HEAT THERAPY FOR CONTROL OF PYTHIUM STORAGE ROT OF BEGONIA TUBERS

R.D. Raabe | K.F. Baker

35. OBSERVATIONS ON SCLEROTINIA (BOTRYTIS) NARCISSICOLA GREG., THE CAUSE OF NARCISSUS SMOULDER IN NORTHERN SCOTLAND

E. G. Gray

36. A POSSIBLE EXPLANATION OF VARIATIONS IN SUSCEPTIBILITY OF TULIP BULBS TO INFECTION BY FUSARIUM OXYSPORUM

B.H.H. Bergman | J.C.M. Beijersbergen

37. ENZYMATIC LIBERATION OF TULIPALIN (α-METHYLENEBUTYROL-ACTONE), A FUNGITOXIC SUBSTANCE ISOLATED FROM TULIPS

J.C.M. Beijersbergen | Cath.B.G. Lemmers

38. THE INFECTION OF BULBS BY PENICILLIUM SP.

G.J. Saaltink

39. BUD NECROSIS IN TULIPS, A MULTIFACTORIAL DISORDER

W.J. de Munk

40. NARCISSUS EELWORM DITYLENCHUS DIPSACI: SOME ASPECTS OF ITS BIOLOGY AND CONTROL BY THIONAZIN

J.J. Hesling

41. CHEMICAL CONTROL OF DITYLENCHUS DIPSACI IN NARCISSUS AND TULIP

N.G.M. Hague | M. Kondrollochis

42. HORIZONTAL MIGRATION OF THE TULIP RACE OF STEM NEMATODE DITYLENCHUS DIPSACI (KUEHN) FROM ARTIFICIALLY INFECTED TULIP BULBS PLANTED ON A SANDY AND A HEAVY CLAY SOIL

W.A. Windrich

43. EXPERIMENTS ON TULIP BULB PRODUCTION IN RELATION TO CUCUMBER MOSAIC VIRUS

M. Yamamoto

44. ASTER YELLOWS IN GLADIOLUS IN THE NETHERLANDS: CURE BY HOT-WATER TREATMENT AND PREVENTION OF SPREAD WITH SYSTEMIC INSECTICIDES

D.H.M. van Slogteren

45. INSTABILITY OF SOME FLEXUOUS ROD-SHAPED VIRUS PARTICLES DURING PURIFICATION*

G.A. de Zoeten | C.E. Fribourg

46. A NEW DISEASE OF TULIP RESEMBLING AUGUSTA DISEASE*

W.P. Mowat

47. ALUMINUM FOIL MULCH REDUCES APHID TRANSMISSION OF CUCUMBER MOSAIC VIRUS IN GLADIOLUS

A. Bing | G.V. Johnson

48. OCCURRENCE AND IMPORTANCE OF VIRUSES INFECTING NARCISSUS IN BRITAIN

A.A. Brunt

49. WEED COMPETITION IN NARCISSUS CROPS

H.M. Lawson

50. THE SELECTIVITY OF SOME HERBICIDES IN BULB GROWING

J.L.P. van Oorschot | M. de Rooy

51. EFFECT OF SIZE AND SHAPE OF TULIP BULBS ON ROOT DEVELOPMENT

J.J. Schuurman

52. TULIP ROOT BEHAVIOUR AND AERATION REQUIREMENTS

L.K. Wiersum

53. EFFECT OF COMPACTION ON PHYSICAL PROPERTIES OF SOIL AND ROOT GROWTH OF ORNAMENTAL BULBS

F.A.M. de Haan | G.G.M. van der Valk

54. INFLUENCE OF SHORT PERIODS OF RESTRICTED SOIL AERATION ON DEVELOPMENT OF TULIPS

G.G.M. van der Valk

55. SOIL STRUCTURE PROBLEMS IN TULIP CULTURE

P. Boekel

56. THE USE OF ORGANIC MATERIALS IN FLOWERBULB GROWING

J.A. van den Berg

57. FROST INJURY TO FLOWERBULB CROPS

G.G.M. van der Valk

58. APPLIED MECHANIZATION OF TULIP-BULB PRODUCTION ON CLAY AND SANDY-CLAY SOILS

J. van den Broek

59. STRUCTURAL PROBLEMS ON BULB HOLDINGS IN THE NETHERLANDS

W.G. de Haan

60. ECONOMIC ASPECTS OF FLOWER PRODUCTION ON BULB-GROWING FARMS

C.O.N. de Vroomen

61. TRENDS IN THE MARKETING AND ‘CONSUMPTION’ OF FLOWER BULBS

N.C. Hofman

62. EXPORT INSPECTION OF FLOWERBULBS IN THE NETHERLANDS

J.A.J. Veenenbos

63. FIELD INSPECTION OF BULBOUS PLANTS IN THE NETHERLANDS

H. van Os

64. BULB GROWING IN POLAND

H. Dargiewicz

65. MECHANIZATION OF ANEMONE CORM PRODUCTION

H.J. Eaton

66. SOME RESULTS OF BREEDING RESEARCH ON TULIPS (TULIPA SP.)

W. Horn

67. PROSPECTS OF PRESELECTION IN TULIP BREEDING

J.P. van Eijk

68. MUTATION BREEDING IN FLOWER BULBS

C. Broertjes | H.Y. Alkema

69. SHORTENING THE PERIOD FROM SEED TO A FLOWERING BULB IN TULIP*

E.J. Fortanier

70. POLYPLOIDY IN FREESIAS

A.E. Zeilinga

71. METHODICAL PROBLEMS IN GLADIOLUS HYBRIDIZATION

A.N. Gromov

72. REGENERATION OF ISOLATED BULB SCALE SEGMENTS OF HYACINTH*

R.L.M. Pierik | J. Woets