Articles
A LINKAGE MAP OF THE TOMATO SPOTTED WILT VIRUS RESISTANCE GENE SW-5 USING NEAR ISOGENIC LINES AND AN INTERSPECIFIC CROSS
Article number
431_33
Pages
385 – 392
Language
Abstract
Restriction fragment length polymorphism (RFLP) analyses has been used to position Sw-5, the tomato spotted wilt virus (TSWV) resistance gene derived from Lycopersicon peruvianum, to the long arm of chromosome 9 of tomato (L. esculentum) in the subtelomeric region between CT71 and CT220. We have identified additional codominant randomly amplified polymorphic DNA (RAPD) markers linked to Sw-5. The RAPD originating from the resistant DNA is a 0.94-kilo base pair (kbp) band, Thereafter termed 421R. This resistant band was determined to be linked to CT71 and Sw-5 in a population segregating for Sw-5. These data were confirmed using the original interspecific population L. esculentum x L. pennellii (LA716) used to map the Sw-5 region.
Susceptible L. esculentum plants lack the 421R band but contain a 0.90-kbp band not present in resistant plants or in TSWV-susceptible L. pennellii plants.
Near isogenic lines (NILs) were developed to aid in the localization of Sw-5. Using a segregating L. esculentum backcross population derived from the NILs we have mapped the 421R band and Sw-5 to about 1 cM. Furthermore, using RFLP analyses on resistant plants from the NIL segregating backcross population, it was determined that CT220 is about 2.4 cM from Sw-5. In addition, using pulse-field gel electrophoresis (PFGE) for restriction mapping of large DNAs it was determined that a probable crossover event separating L. esculentum from L. peruvianum is between CT71 and Sw-5. The evidence also suggests that CT220 maps within the introgressed L. peruvianum DNA containing Sw-5.
Susceptible L. esculentum plants lack the 421R band but contain a 0.90-kbp band not present in resistant plants or in TSWV-susceptible L. pennellii plants.
Near isogenic lines (NILs) were developed to aid in the localization of Sw-5. Using a segregating L. esculentum backcross population derived from the NILs we have mapped the 421R band and Sw-5 to about 1 cM. Furthermore, using RFLP analyses on resistant plants from the NIL segregating backcross population, it was determined that CT220 is about 2.4 cM from Sw-5. In addition, using pulse-field gel electrophoresis (PFGE) for restriction mapping of large DNAs it was determined that a probable crossover event separating L. esculentum from L. peruvianum is between CT71 and Sw-5. The evidence also suggests that CT220 maps within the introgressed L. peruvianum DNA containing Sw-5.
Authors
M.R. Stevens, D.K. Heiny, D.D. Rhoads, P.D. Griffiths, J.W. Scott
Keywords
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