Articles
GENETICALLY ENGINEERED RESISTANCE TO TOMATO SPOTTED WILT VIRUS IN CHRYSANTHEMUM (DENDRANTHEMA GRANDIFLORA): A MODEL SYSTEM FOR VIRUS PROTECTION IN ORNAMENTAL CROPS
Article number
431_39
Pages
432 – 441
Language
Abstract
We have employed chrysanthemum as a model system for exploring the feasibility of using genetically engineered resistance to tomato spotted wilt virus (TSWV) in ornamental crops.
This approach was taken because TSWV has become a major problem for ornamental production and current control measures lack effectiveness.
We have developed an efficient Agrobacterium-mediated transformation system that allows recovery of transgenic plants from three cultivars of chrysanthemum.
We have transformed the highly TSWV-susceptible chrysanthemum cultivar Polaris with the TSWV nucleocapsid (N) protein gene.
Resistant plants were identified following challenge by viruliferous thrips that vectored a highly virulent heterologous isolate of TSWV. This paper documents the first time an ornamental crop has been engineered with TSWV-resistance.
This approach was taken because TSWV has become a major problem for ornamental production and current control measures lack effectiveness.
We have developed an efficient Agrobacterium-mediated transformation system that allows recovery of transgenic plants from three cultivars of chrysanthemum.
We have transformed the highly TSWV-susceptible chrysanthemum cultivar Polaris with the TSWV nucleocapsid (N) protein gene.
Resistant plants were identified following challenge by viruliferous thrips that vectored a highly virulent heterologous isolate of TSWV. This paper documents the first time an ornamental crop has been engineered with TSWV-resistance.
Authors
John M. Sherman, James W. Moyer, M. E. Daub
Keywords
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